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| Barros,Gustavo A. C.; Pereira,Andreia V.; Barros,Luciana C.; Lourenço Jr,Airton; Calvi,Sueli A.; Santos,Lucilene D.; Barraviera,Benedito; Ferreira Jr,Rui Seabra. |
| Abstract Background American visceral leishmaniasis is caused by the intracellular parasiteLeishmania (L.) infantum chagasi, and transmitted by the sand fly Lutzomyia longipalpis. Since treatment is based on classical chemotherapeutics with significant side effects, the search for new drugs remains the greatest global challenge. Thus, this in vitro study aimed to evaluate the leishmanicidal effect ofCrotalus durissus terrificus venom fractions on promastigote and amastigote forms of Leishmania (L.) infantum chagasi. Methods Phospholipase A 2 (PLA 2 ) and a pool of peptide fraction (<3 kDa) were purified from Crotalusvenom. Furthermore, promastigotes and peritoneal macrophages of mice infected by amastigotes were exposed to serial dilutions of the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: PLA2; Peptides; Crotalus dutissus terrificus; Venom; Leishmanicidal activity. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100351 |
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| Rodríguez,JP; De Marzi,MC; Maruñak,S; Teibler,P; Acosta,O; Malchiodi,EL; Leiva,LC. |
| We examined the ability of IgG anti-crotalic PLA2 to cross-react with Bothrops spp. venoms, from snakes found in the northeast of Argentina. Immunoblotting and ELISA tests showed that IgG anti-crotalic PLA2 recognize antigens of bothropic venoms. Indirect hemolytic activity tests showed that the quantity of antibodies that neutralized 50% of Crotalus durissus terrificus venom (ED50: 2.1 mg IgG anti-crotalic PLA2/100 µg of venom) were also able to neutralize venom from other snakes in the following proportion: 34% of B. alternatus, 18% of B. diporus and 12% of B. jararacussu. Likewise, direct PLA2 activity neutralization tests showed a similar cross-neutralization pattern including 56% of B. alternatus, 29% of B. diporus and 30% of B. jararacussu. In... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bothrops; Crotalus; PLA2; Neutralization; Cross-reaction. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000300008 |
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