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	Provedor de dados BJMBR (1) Ciência Rural (1) Genet. Mol. Biol. (1) Autor Abreu,R.B.V. (1) Araújo Júnior,João Pessoa (1) Freitas-Alves,D.R. (1) Harada,Maria Lucia (1) Kurissio,Jacqueline Kazue (1) Nascimento Borges,Barbara do (1) Piranda,D.N. (1) Rodriguez Burbano,Rommel Mario (1) Rosário Pinheiro,Danilo do (1) Vianna-Jorge,R. (1) de Carvalho,M.A. (1) Mais... Palavra-chave PTGS2 (3) Animal model (1) COX-2 (1) COX2 (1) CaHV-1 (1) Canine pathogen (1) Gastric cancer (1) Gene expression (1) Gene regulation (1) HEK293FT (1) Haplotypes (1) Herpesvirus (1) MCF-7 (1) Transcriptome (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Ano 2018 (3) País Brazil (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Modulation of the prostaglandin-endoperoxide synthase 2 gene expression by variant haplotypes: influence of the 3′-untranslated region BJMBR Piranda,D.N.; Abreu,R.B.V.; Freitas-Alves,D.R.; de Carvalho,M.A.; Vianna-Jorge,R.. The inducible inflammatory enzyme cycloxigenase-2 is up-regulated in cancer, and favors tumor progression. Cycloxigenase-2 is encoded by the prostaglandin-endoperoxide synthase 2 (PTGS2) gene, which presents sequence variations in the promoter region (PR) and in the 3′-untranslated region (3′-UTR). Different PR (rs689465, rs689466, rs20417) and 3′-UTR (rs5275) variants were generated by site-directed mutagenesis, and combined in haplotypes to access expression levels using a reporter system (luciferase) in human cells (MCF-7 and HEK293FT). Luciferase activity did not differ significantly among PTGS2 PR constructs, except for pAAC (containing variant allele rs20417 C), with 40% less activity than pAAG (wild-type sequence) in MCF-7 cells (P<0.01). Despite... Tipo: Info:eu-repo/semantics/article Palavras-chave: COX-2; PTGS2; Haplotypes; Gene expression; MCF-7; HEK293FT. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000200602 Transcriptomic analysis of differential gene expression reveals an increase in COX2 levels during in vitro canine herpesvirus infection Ciência Rural Kurissio,Jacqueline Kazue; Araújo Júnior,João Pessoa. ABSTRACT: Canine herpesvirus (CaHV-1) affects canids worldwide, causing death in neonates and immunosuppressed hosts. Acute infection by CaHV-1 can cause reproductive, respiratory, and neurological problems in adult animals. Viral pathogenesis and host genes expressions during of CaHV-1infection are not clearly understood. In the present study, the transcriptome of canine kidney cell Mardin-Darby (MDCK) infected in vitro with canine herpesvirus was explored. For this, RNA sequencing (RNA-seq) of the samples in different moments during infection was carried out. Subsequently, the transcriptomic analysis genes related to cell activities and process involved to viral cycle infection were evaluated until 32h post-inoculation (pi). Among evaluated genes, was... Tipo: Info:eu-repo/semantics/article Palavras-chave: Transcriptome; Herpesvirus; CaHV-1; PTGS2; COX2; Canine pathogen. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001000451 COX-2 gene expression and methylation profile in Sapajus apella as an experimental model for gastric adenocarcinoma Genet. Mol. Biol. Rosário Pinheiro,Danilo do; Harada,Maria Lucia; Rodriguez Burbano,Rommel Mario; Nascimento Borges,Barbara do. Abstract Gastric cancer (GC) remains one of the main causes of cancer-related death worldwide. There are two distinct histological types of GC: diffuse and intestinal. The latter is characterized by the presence of pre-neoplastic lesions. One of the most frequently altered enzymes in intestinal GC is COX-2, an important lesion marker. This work aimed to study COX-2 methylation and expression in N-methyl-N-Nitrosurea (MNU)-induced intestinal GC in six Sapajus apella animals. The partial promoter sequence of S. apella COX-2 gene was obtained and used to identify transcription factors and cis-regulatory element binding sites. The COX-2 methylation pattern was assessed using Methylation-Specific PCR (MSP), and expression was analyzed by immunohistochemistry... Tipo: Info:eu-repo/semantics/article Palavras-chave: PTGS2; Gene regulation; Animal model; Gastric cancer. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000300496 Registros recuperados: 3 Primeira ... 1 ... Última

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