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Identification of Polyunsaturated Fatty Acids Synthesis Pathways in the Toxic Dinophyte Alexandrium minutum Using 13C-Labelling ArchiMer
Remize, Marine; Planchon, Frederic; Loh, Ai Ning; Le Grand, Fabienne; Lambert, Christophe; Bideau, Antoine; Bidault, Adeline; Corvaisier, Rudolph; Volety, Aswani; Soudant, Philippe.
The synthetic pathways responsible for the production of the polyunsaturated fatty acids 22:6n-3 and 20:5n-3 were studied in the Dinophyte Alexandrium minutum. The purpose of this work was to follow the progressive incorporation of an isotopic label (13CO2) into 11 fatty acids to better understand the fatty acid synthesis pathways in A. minutum. The Dinophyte growth was monitored for 54 h using high-frequency sampling. A. minutum presented a growth in two phases. A lag phase was observed during the first 30 h of development and had been associated with the probable temporary encystment of Dinophyte cells. An exponential growth phase was then observed after t30. A. minutum rapidly incorporated 13C into 22:6n-3, which ended up being the most 13C-enriched...
Tipo: Text Palavras-chave: PUFA synthesis; 22:6n-3; DHA; PKS pathway; Dinophyte; Alexandrium minutum; Encystment.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00654/76562/77688.pdf
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