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Castro,André L. M. de; Vollú,Renata E.; Peixoto,Raquel S.; Grigorevski-Lima,André L.; Coelho,Rosalie R. R.; Bon,Elba P. S.; Rosado,Alexandre S.; Seldin,Lucy. |
A cellulolytic bacterial strain, designated P118, isolated from the gut of the tropical fish Parotocinclus maculicauda was identified as belonging to the genus Paenibacillus based on phenotypic and chemotaxonomic characteristics and the 16S rRNA gene sequence. The novel strain was Gram-positive, spore-forming and rod-shaped. Catalase but not oxidase was produced. Carboxymethylcellulose was hydrolyzed but starch or gelatin was not. Acetoin production was negative whereas nitrate reduction and urease production were positive. Many carbohydrates served as carbon sources for growth. MK-7 was the predominant isoprenoid quinone. Anteiso-C15:0 (38.73%) and C16:0 (20.85%) were the dominant cellular fatty acids. Strain P118 was closely related to Paenibacillus... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bioprospection; Cellulose degradation; Paenibacillus sp.; Parotocinclus maculicauda; Taxonomy. |
Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400048 |
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Oliveira,Patrícia Lopes de; Duarte,Marta Cristina Teixeira; Ponezi,Alexandre Nunes; Durrant,Lúcia Regina. |
The production of manganese peroxidase (MnP) from Bacillus pumilus and Paenibacillus sp. was studied under absence and presence of the inducers indulin AT, guayacol, veratryl alcohol, lignosulfonic acid and lignosulfonic acid desulfonated. Indulin AT increased the activity of B. pumilus MnP up to 31.66 U/L after 8 h, but no improve was observed for Paenibacillus sp., which reached maximum activity (12.22 U/L) after 20 h. Both MnPs produced by these microorganisms were purified in phenyl sepharose resin and the proteins from crude extracts were eluted in two fractions. However, only the first fraction of each extract exhibited MnP activities. Tests in different pH and temperature values, from pH 5.0 to pH 10.0 and 30 ºC to 60 ºC, respectively, were carried... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacillus pumilus; Paenibacillus sp.; Manganese peroxidase; Purification; Characterization. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400012 |
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