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| Registros recuperados: 34 | |
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| MENEZES,FRANCISCA G.R. DE; RODRIGUEZ,MARINA T.T.; CARVALHO,FÁTIMA C.T. DE; REBOUÇAS,ROSA H.; COSTA,RENATA A.; SOUSA,OSCARINA V. DE; HOFER,ERNESTO; VIEIRA,REGINE H.S.F.. |
| ABSTRACT Detection of virulent strains associated with aquatic environment is a current concern for the management and control of human and animal health. Thus, Vibrio diversity was investigated in four estuaries from state of Ceará (Pacoti, Choró, Pirangi and Jaguaribe) followed by antimicrobial susceptibility to different antimicrobials used in aquaculture and detection of main virulence factors to human health. Isolation and identification were performed on TCBS agar (selective medium) and dichotomous key based on biochemical characteristics, respectively. Nineteen strains of genus Vibrio were catalogued. Vibrio parahaemolyticus (Choró River) and V. alginolyticus (Pacoti River) were the most abundant species in the four estuaries. All strains were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Vibrio spp.; Pathogen; Estuaries; Public health; Water. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000301175 |
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| Gomes,Jorge Augusto Assis; Andrade Júnior,Valter Carvalho; Oliveira,Celso Mattes de; Azevedo,Alcinei Mistico; Maluf,Wilson Roberto; Gomes,Luiz Antonio Augusto. |
| The objective of this study was to evaluate sweet potato clones for resistance to root-knot nematode, Meloidogyne incognita races 1 and 3. For each nematode physiological race, a greenhouse experiment was set up in a randomized block experimental design with three replications and six plants per plot. Fifty-eight sweet potato clones from the UFVJM germplasm bank were evaluated, plus five commercial cultivars (Brazlândia Rosada, Brazlândia Branca, Palmas, Princesa and Coquinho), plus the cv. Santa Clara tomato (susceptible to Meloidogyne spp.). Stems were planted in 72-cell expanded polystyrene trays, filled with commercial substrate and inoculated with the pathogen thirty days after planting. Forty-five days after inoculation, the eggs were extracted,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ipomoea batatas; Root-knot nematode; Breeding; Pathogen; Reproduction factor; Reproduction index. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052015000300291 |
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| Martins,José M.; Galinari,Éder; Pimentel-Filho,Natan J.; Ribeiro Jr,José I.; Furtado,Mauro M.; Ferreira,Célia L.L.F.. |
| Physical, physicochemical, and microbiological changes were monitored in 256 samples of artisanal Minas cheese from eight producers from Serro region (Minas Gerais, Brazil) for 64 days of ripening to determine the minimum ripening time for the cheese to reach the safe microbiological limits established by Brazilian legislation. The cheeses were produced between dry season (April–September) and rainy season (October–March); 128 cheeses were ripened at room temperature (25 ± 4 °C), and 128 were ripened under refrigeration (8 ± 1 °C), as a control. No Listeria monocytogenes was found, but one cheese under refrigeration had Salmonella at first 15 days of ripening. However, after 22 days, the pathogen was not detected. Seventeen days was the minimum ripening... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ripening; Artisanal Minas cheese; Serro; Pathogen; Lactic acid bacteria. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100219 |
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| Farooq,Muzammil; Freed,Shoaib. |
| Abstract The housefly Musca domestica is a worldwide insect pest that acts as a vector for many pathogenic diseases in both people and animals. The present study was conducted to evaluate the virulence of different local isolates of Beauveria bassiana, Metarhizium anisopliae and Isaria fumosorosea on M. domestica using two bioassay techniques: (1) adult immersion and (2) a bait method applied to both larvae and adults. The results showed evidence of a broad range of responses by both stages (larvae and adults) to the tested isolates of B. bassiana, M. anisopliae and I. fumosorosea. These responses were concentration-dependent, with mortality percentages ranging from 53.00% to 96.00%. Because it resulted in lower LC50 values and a shorter lethal time, B.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Entomopathogenic fungi; Fecundity; Housefly; Infectivity; Pathogen; Vector. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000400807 |
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| Mendonça,Karla Sequeira; Michael,Geovana Brenner; Nalério,Élen Silveira; Mendonça,Marcelo; Cardoso,Marisa Ribeiro Itapema; Silva,Wladimir Padilha da. |
| ABSTRACT: Listeria monocytogenes is of notable concern to the food industry, due to its ubiquitous nature and ability to grow in adverse conditions. This study aimed to determine the genotypic profile of L. monocytogenes strains isolated from refrigerated chickens marketed in the southern part of Rio Grande do Sul, Brazil. The strains of L. monocytogenes isolated were characterized by serotyping and Pulsed Field Gel Electrophoresis (PFGE). Three different serotypes (1/2a, 1/2b and 4e) were evaluated by PFGE, and the macrorestriction patterns utilizing enzymes AscI and ApaI, revealed five different pulsotypes. The presence of such varied genotypic profiles demonstrates the prevalence of L. monocytogenes contamination of chicken processing environments,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Contamination; Pathogen; Prevalence; Serotyping. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000100132 |
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| Cumming, Graeme S.; Percy FitzPatrick Institute, DST/NRF Center of Excellence, University of Cape Town, Rondebosch, 7701, South Africa; graeme.cumming@uct.ac.za; Hockey, Philip A. R.; Percy FitzPatrick Institute, DST/NRF Center of Excellence, University of Cape Town, Rondebosch, 7701, South Africa; phil.hockey@uct.ac.za; Bruinzeel, Leo W.; Percy FitzPatrick Institute, DST/NRF Center of Excellence, University of Cape Town, Rondebosch, 7701, South Africa; lbruinze@adu.uct.ac.za; Du Plessis, Morne A.; Percy FitzPatrick Institute, DST/NRF Center of Excellence, University of Cape Town, Rondebosch, 7701, South Africa; mornedup@vodamail.co.za. |
| Global analyses of the potential for avian influenza transmission by wild birds have ignored key characteristics of the southern African avifauna. Although southern Africa hosts a variety of migratory, Holarctic-breeding wading birds and shorebirds, the documented prevalence of avian influenza in these species is low. The primary natural carriers of influenza viruses in the northern hemisphere are the anatids, i.e., ducks. In contrast to Palearctic-breeding species, most southern African anatids do not undertake predictable annual migrations and do not follow migratory flyways. Here we present a simple, spatially explicit risk analysis for avian influenza transmission by wild ducks in southern Africa. We developed a risk value for each of 16 southern... |
| Tipo: Peer-Reviewed Reports |
Palavras-chave: Anatidae; Avian influenza; Botswana; Ducks; Influenza; Landscape ecology; Namibia; Pathogen; South Africa; Virus; Waterfowl; Zimbabwe.. |
| Ano: 2008 |
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| Cumming, Graeme S.; Percy FitzPatrick Institute, DST/NRF Centre of Excellence, University of Cape Town; graeme.cumming@uct.ac.za. |
| Pathogen dynamics are inseparable from the broader environmental context in which pathogens occur. Although some pathogens of people are primarily limited to the human population, occurrences of zoonoses and vector-borne diseases are intimately linked to ecosystems. The emergence of these diseases is currently being driven by a variety of influences that include, among other things, changes in the human population, long-distance travel, high-intensity animal-production systems, and anthropogenic modification of ecosystems. Anthropogenic impacts on ecosystems have both direct and indirect (food-web mediated) effects. Therefore, understanding disease risk for zoonoses is a social–ecological problem. The articles in this special feature focus on... |
| Tipo: Peer-Reviewed Reports |
Palavras-chave: Disease; Framework; Health; Influenza; Pathogen; Resilience; Social– Ecological system. |
| Ano: 2010 |
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| Oliveira,Ademir Kleber M; Rizzi,Elvia Silvia; Pereira,Kelly Cristina L; Bono,José Antônio M; Matias,José Carlos Pina; Rosemary. |
| ABSTRACT The use of plant extracts can be an alternative to reduce the use of pesticides, to control diseases and pests that affect agricultural production. The aim of this study was the chemical characterization of the extract and fractions of Pouteria ramiflora leaves and to determine its fungistatic potential on Lasiodiplodia theobromae. The ethanolic extract of dried and crushed leaves was obtained by maceration. Part of this extract was sequentially partitioned and the following fractions were obtained: hexane, dichloromethane, chloroform, ethyl acetate, n-butyl, and hydromethanol for subsequent bioassay, and qualitative and quantitative phytochemical analysis. Experimental development comprises of adding a L. theobromae mycelial disk (5 mm), obtained... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pathogen; Natural products; Organic farming; Flavonoids. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362017000400564 |
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| Garnier, Matthieu; Labreuche, Yannick; Nicolas, Jean-louis. |
| Eleven Vibrio isolates invading the hemolymph of live and moribund oysters (Crassostrea gigas) collected in the field and from a hatchery in France, were characterized by a polyphasic approach. Phylogenetic analysis of 16S rRNA, gyrB and toxR genes indicated high homogeneity between these strains and the Vibrio aestuarianus type strain (ATCC35048(T)), and confirmed previous 16S rRNA analysis. In contrast, DNA:DNA hybridization was from 61% to 100%, while phenotypic characters and virulence tests showed a large diversity between the strains. Nevertheless, several common characters allowed the isolates to be distinguished from the reference strain. On the basis of several distinct phenotypic characteristics, it is proposed to establish two subspecies within... |
| Tipo: Text |
Palavras-chave: Polyphasic approach; Summer mortalities; Crassostrea gigas; Pathogen; French isolates; Vibrio aestuarianus. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4961.pdf |
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| Goarant, Cyrille; Reynaud, Yann; Ansquer, Dominique; De Decker, Sophie; Merien, Fabrice. |
| In a previous study, we demonstrated the existence of an emerging cluster of Vibrio nigripulchritudo that proved to be associated with shrimp mortality events in New Caledonia. Using sequence polymorphisms evidenced in this previous MultiLocus Sequence Typing study, we developed two new quantitative PCR assays permitting the detection and quantification of V. nigripulchritudo at the genospecies level using SYBR Green I chemistry and at the emerging cluster level using Fluorescence Resonance Energy Transfer technology with hybridization probes. The use of this molecular diagnostic tool evidenced the colonization of the shrimp pond ecosystem by the pathogenic cluster at least at the onset of the disease. This new tool will allow better investigation of the... |
| Tipo: Text |
Palavras-chave: Shrimp; Cluster specific; Vibrio; Pathogen; Mariculture; Real time PCR. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2729.pdf |
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| Lassalle, Damien; Tetreau, Guillaume; Pinaud, Silvain; Galinier, Richard; Crickmore, Neil; Gourbal, Benjamain; Duval, David. |
| Biomphalaria glabrata is a freshwater Planorbidae snail. In its environment, this mollusk faces numerous microorganisms or pathogens, and has developed sophisticated innate immune mechanisms to survive. The mechanisms of recognition are quite well understood in Biomphalaria glabrata, but immune effectors have been seldom described. In this study, we analyzed a new family of potential immune effectors and characterized five new genes that were named Glabralysins. The five Glabralysin genes showed different genomic structures and the high degree of amino acid identity between the Glabralysins, and the presence of the conserved ETX/MTX2 domain, support the hypothesis that they are pore-forming toxins. In addition, tertiary structure prediction confirms that... |
| Tipo: Text |
Palavras-chave: Pore-forming toxin; Cry toxin; Invertebrate; Biomphalaria glabrata; Host; Pathogen; Vector snail; Innate immunity. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00601/71267/69655.pdf |
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| Le Guyader, Soizick; Atmar, Robert; Maalouf, Haifa; Le Pendu, Jacques. |
| Shellfish can be a vector for human pathogens. Despite regulation based on enteric bacteria, shellfish are still implicated in viral outbreaks. Oysters are the most common shellfish associated with outbreaks, and noroviruses, which cause acute gastroenteritis, are the most frequently identified pathogen in these outbreaks. Analysis of shellfish-related outbreak data worldwide shows an unexpected high proportion of NoV GI strains. Recent studies performed in vitro, in vivo and in the environment indicate that oysters are not just a passive filter, but can selectively accumulate norovirus strains based on virus carbohydrate ligands shared with humans. These observations may help explain the GI/GII bias observed in shellfish-related outbreaks compared to... |
| Tipo: Text |
Palavras-chave: Shellfish; Noroviruses; Oyster contamination; Pathogen. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00150/26093/24313.pdf |
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| Registros recuperados: 34 | |
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