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| Orce,Ingrid Georgina; Sendín,Lorena Noelia; Marano,María Rosa; Vojnov,Adrián Alberto; Castagnaro,Atilio Pedro; Filippone,María Paula. |
| Huanglongbing (HLB), a devastating citrus disease caused by the bacterium “Candidatus Liberibacter spp.”, is now responsible for significant economic losses worldwide. Yet, no effective disease control has been found, and the non-cultivability of the bacterium has severely hampered studies on the pathogen. The 16S rDNA gene is a well-characterized sequence, essential for cell survival, and is used for bacterial identification or assignment of close relationships at the genus and species levels. Quantitative Real-Time PCR (qPCR) assays based on 16S rDNA genes are widely used in the detection of “Ca. Liberibacter spp.” in multiplex reactions. We have developed for the first time a set of qPCR primers based on the conserved 16S rDNA gene, which specifically... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Candidatus Liberibacter spp.; Syber Green qPCR; Argentina; Greening; Pathogen detection. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162015000300252 |
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| Nameth,S.T.. |
| Seed pathology as a subdisipline of plant pathology is relatively new. Paul Neergard is considered the father of seed pathology. Recent developments in the area of seed pathology technology allow for more ecofriendly seed treatments and more reliable seed health testing. Due to economics and new interest in environmental issues, research into the viability of biological seed treatments is becoming more common. The use of sophisticated DNA amplification technologies allows for the detection of seedborne pathogens that might go undetected using more conventional means. These types of research will be fundamental in guaranteeing seed health quality standards and achieving phytosanitary requirements throughout the world in the new millennium. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Seedborne pathogens; Pathogen detection; Seed treatments; PCR; DNA amplification. |
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161998000500017 |
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