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| Oliveira,Rodrigo de Queiroz; Valasques Junior,Gildomar Lima; Uetanabaro,Ana Paula Trovatti; Koblitz,Maria Gabriela Bello; Góes-Neto,Aristóteles; Rosa,Carlos Augusto; Assis,Sandra Aparecida de. |
| Microbial pectinolytic enzymes are known to play a commercially important role in a number of industrial processes. Two kinds of yeast can be discerned regarding the production of enzymes. One group includes those which can produce enzymes in the absence of an inducer, and the other group comprises the yeasts that produce enzymes in the presence of an inducer. The objective of this study was to investigate the influence of pectic substances, glucose, pH, and temperature on the polygalacturonase activity by Kluyveromyces marxianus CCMB 322. The yeast was grown in a fermentation broth containing different concentrations of glucose and pectic substances. The polygalacturonase activity was determined by the DNS method, and the pH and temperature were optimized... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Yeasts; Enzymes; Thermostability; Pectic substances; Biotechnology. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612012000300012 |
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| Vitorino,Patrícia Goulart; Alves,José Donizeti; Magalhães,Paulo César; Magalhães,Marcelo Murad; Lima,Luíz Carlos Oliveira; Oliveira,Luiz Edson Mota de. |
| This research aimed to characterize the tolerance to flooding and alterations in pectic and hemicellulose fractions from mesocotyl of maize tolerant to flooding when submitted to hypoxia. In order to characterize tolerance seeds from maize cultivars Saracura BRS-4154 and BR 107 tolerant and sensitive to low oxygen levels, respectively, were set to germinate. Plantlet survival was evaluated during five days after having been submitted to hypoxia. After fractionation with ammonium oxalate 0.5% (w/v) and KOH 2M and 4M, Saracura BRS-4154 cell wall was obtained from mesocotyl segments with different damage intensities caused by oxygen deficiency exposure. The cell wall fractions were analyzed by gel filtration and gas chromatography, and also by Infrared... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pectic substances; Hemicellulose. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2001000800004 |
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