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Penicillin production by wild isolates of Penicillium chrysogenum in Pakistan 58
Rahman,Sajjad-Ur; Rasool,Muhammad Hidayat; Rafi,Muhammad.
The present study was aimed at exploring the native wild isolates of Penicillium chrysogenum series in terms of their penicillin production potential. Apart from the standard medium, the efforts were made to utilize suitable agro-industrial wastes for the maximum yield of penicillin. Two series of P. chrysogenum were isolated from local sources and named as P. chrysogenum series UAF R1 and P. chrysogenum series UAF R2. The native series were found to possess better penicillin production potential than the already reported series of P. chrysogenum. However, P. chrysogenum series UAF R1 was found to be the best candidate for high yield of penicillin starting at 100 hour as compared to P. chrysogenum series UAF R2 which produced the highest yield of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Penicillin; Penicillium chrysogenum; Corn Steep Liquor; Sugar Cane Bagasse.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200007
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Screening and genetic improvement of pectinolytic fungi for degumming of textile fibers 58
Molina,Silvia M.G.; Pelissari,Francisco A.; Vitorello,Cláudia B.M..
Aiming at contributing to technological improvements in plant fiber processing methods, this paper reports research work on the obtainment of more efficient pectinase-producing fungi strains. More specifically, this work reports the analysis of 18 strains of filamentous fungi, with the purpose of obtaining enzymes for textile fibers degumming. The strains were evaluated for production of pectinolytic enzymes under several growth conditions (culture medium and growth temperature). Production of pectinases was measured by an enzymatic index (EI) in solid pectin medium. Among the tested strains, Penicillium chrysogenum IFO 4626 (Q 176) showed the best performance. Genetic improvement of this strain was carried out to increase its pectinase production, while...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Penicillium chrysogenum; Pectin lyase; Pectinolytic enzyme; Degumming of textile fibers.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000400013
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Yeast HXK2 gene reverts glucose regulation mutation of penicillin biosynthesis in P. chrysogenum 58
Pérez,Edmundo A.; Fernández,Francisco J.; Fierro,Francisco; Mejía,Armando; Marcos,Ana T.; Martín,Juan F.; Barrios-González,Javier.
The mutant Penicillium chrysogenum strain dogR5, derived from strain AS-P-78, does not respond to glucose regulation of penicillin biosynthesis and β-galactosidase, and is partially deficient in D-glucose phosphorilating activity. We have transformed strain dogR5 with the (hexokinase) hxk2 gene from Saccharomyces cerevisiae. Transformants recovered glucose control of penicillin biosynthesis in different degrees, and acquired a hexokinase (fructose phosphorylating) activity absent in strains AS- P-78 and dogR5. Interestingly, they also recovered glucose regulation of β-galactosidase. On the other hand, glucokinase activity was affected in different ways in the transformants; one of which showed a lower activity than the parental dogR5, but normal glucose...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glucose regulation; Glucokinase; Penicillin; Penicillium chrysogenum.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300017
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