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	Provedor de dados Genet. Mol. Biol. (2) BJM (1) Rev. Microbiol. (1) Autor Araújo,Elza F. de (2) Silva,Daison Olzany (2) Coelho,Jorge Luiz Cavalcante (1) Gonçalves,Daniel B. (1) Minussi,Rosana Cristina (1) Passos,Flávia Maria Lopes (1) Passos,Frederico José Vieira (1) Piccoli-Valle,Roberta Hilsdorf (1) Queiroz,Marisa V. (1) Queiroz,Marisa V. de (1) Ribeiro,João Batista (1) Ribon,Andréa O. B. (1) Ribon,Andréa de O.B. (1) Soares-Ramos,Juliana Rocha Lopes (1) Mais... Palavra-chave Penicillium griseoroseum (4) Polygalacturonase (3) Pectin lyase (2) Pectinases (2) 5' upstream regulatory sequence (1) Electrophoretic mobility shift assay (1) Fermentation (1) Filamentous fungi (1) Gene organization (1) Pectinase (1) Sugar-cane juice (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Info:eu-repo/semantics/other (1) Ano 2009 (1) 2002 (1) 2001 (1) 1998 (1) País Brazil (4) Idioma Inglês (4)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 4 Primeira ... 1 ... Última Production of pectin lyase by Penicillium griseoroseum in bioreactors in the absence of inducer BJM Piccoli-Valle,Roberta Hilsdorf; Passos,Flávia Maria Lopes; Passos,Frederico José Vieira; Silva,Daison Olzany. Penicillium griseoroseum was grown in bioreactors on mineral medium supplemented with yeast extract and sucrose. The influence of inoculum and carbon source concentrations, aeration and pH on pectin lyase (PL) production, as well as the capacity of P. griseoroseum to produce PL when grown on sugar cane syrup as carbon source were evaluated. Inoculum concentration did not influence PL production. Production was higher in non-aerated than in aerated medium. The best results were obtained using 60 mM sucrose at pH 6.3-7.2. Production using cane syrup 25% (v/v), without yeast extract supplement, was equal to that obtained under the conditions cited above. Tipo: Info:eu-repo/semantics/article Palavras-chave: Fermentation; Pectin lyase; Penicillium griseoroseum; Pectinases. Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000200013 Gel mobility shift scanning of pectin-inducible promoter from Penicillium griseoroseum reveals the involvement of a CCAAT element in the expression of a polygalacturonase gene Genet. Mol. Biol. Ribon,Andréa de O.B.; Ribeiro,João Batista; Gonçalves,Daniel B.; Queiroz,Marisa V. de; Araújo,Elza F. de. Previous reports have described pgg2, a polygalacturonase-encoding gene of Penicillium griseoroseum, as an attractive model for transcriptional regulation studies, due to its high expression throughout several in vitro growth conditions, even in the presence of non-inducing sugars such as sucrose. A search for regulatory motifs in the 5' upstream regulatory sequence of pgg2 identified a putative CCAAT box that could justify this expression profile. This element, located 270 bp upstream of the translational start codon, was tested as binding target for regulatory proteins. Analysis of a 170 bp promoter fragment by electrophoretic mobility shift assay (EMSA) with nuclear extracts prepared from mycelia grown in pectin-containing culture medium revealed a high... Tipo: Info:eu-repo/semantics/other Palavras-chave: Penicillium griseoroseum; Polygalacturonase; 5' upstream regulatory sequence; Electrophoretic mobility shift assay. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100019 Structural organization of polygalacturonase-encoding genes from Penicillium griseoroseum Genet. Mol. Biol. Ribon,Andréa O. B.; Queiroz,Marisa V.; Araújo,Elza F. de. The pectinolytic system of Penicillium griseoroseum has been studied as a model to investigate aspects of gene organization in filamentous fungi. Here we show that the endopolygalacturonase-coding genes previously isolated exist as single copies in the fungus genome. DNA blot analysis revealed the presence of corresponding genes in other Penicillium species, although only one or two genes were found in opposition to the endoPG gene family reported for other filamentous fungi. The nucleotide and amino acid sequences of Penicillium PG genes of retrieved from data banks were compared for intron length and number, codon usage, and consensus sequences for translation initiation sites. The introns are conserved in the same position, although there was no... Tipo: Info:eu-repo/semantics/article Palavras-chave: Penicillium griseoroseum; Filamentous fungi; Pectinase; Polygalacturonase; Gene organization. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400020 Sugar-cane juice induces pectin lyase and polygalacturonase in Penicillium griseoroseum Rev. Microbiol. Minussi,Rosana Cristina; Soares-Ramos,Juliana Rocha Lopes; Coelho,Jorge Luiz Cavalcante; Silva,Daison Olzany. The use of other inducers as substitutes for pectin was studied aiming to reduce the production costs of pectic enzymes. The effects of sugar-cane juice on the production of pectin lyase (PL) and polygalacturonase (PG) by Penicillium griseoroseum were investigated. The fungus was cultured in a mineral medium (pH 6.3) in a rotary shaker (150 rpm) for 48 h at 25oC. Culture media were supplemented with yeast extract and sucrose or sugar-cane juice. Sugar-cane juice added singly to the medium promoted higher PL activity and mycelial dry weight when compared to pectin and the use of sugar-cane juice and yeast extract yielded levels of PG activity that were similar to those obtained with sucrose-yeast extract or pectin. The results indicated that, even at low... Tipo: Info:eu-repo/semantics/article Palavras-chave: Pectin lyase; Polygalacturonase; Pectinases; Penicillium griseoroseum; Sugar-cane juice. Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141998000400002 Registros recuperados: 4 Primeira ... 1 ... Última

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