|
|
|
|
|
| Igoillo-Esteve,Mariana; Maugeri,Dante; Stern,Ana L.; Beluardi,Paula; Cazzulo,Juan J.. |
| Trypanosoma cruzi is highly sensitive to oxidative stress caused by reactive oxygen species. Trypanothione, the parasite's major protection against oxidative stress, is kept reduced by trypanothione reductase, using NADPH; the major source of the reduced coenzyme seems to be the pentose phosphate pathway. Its seven enzymes are present in the four major stages in the parasite's biological cycle; we have cloned and expressed them in Escherichia coli as active proteins. Glucose 6-phosphate dehydrogenase, which controls glucose flux through the pathway by its response to the NADP/NADPH ratio, is encoded by a number of genes per haploid genome, and is induced up to 46-fold by hydrogen peroxide in metacyclic trypomastigotes. The genes encoding... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Trypanosoma cruzi; Chagas disease; Pentose phosphate pathway; Oxidative stress; NADPH generation. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652007000400007 |
| |
|
|
| Misiti,F.; Giardina,B.; Mordente,A.; Clementi,M.E.. |
| Anthracyclines, a class of antitumor drugs widely used for the treatment of solid and hematological malignancies, cause a cumulative dose-dependent cardiac toxicity whose biochemical basis is unclear. Recent studies of the role of the metabolites of anthracyclines, i.e., the alcohol metabolite doxorubicinol and aglycone metabolites, have suggested new hypotheses about the mechanisms of anthracycline cardiotoxicity. In the present study, human red blood cells were used as a cell model. Exposure (1 h at 37ºC) of intact human red blood cells to doxorubicinol (40 µM) and to aglycone derivatives of doxorubicin (40 µM) induced, compared with untreated red cells: i) a ~2-fold stimulation of the pentose phosphate pathway (PPP) and ii) a marked inhibition of the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Anthracyclines; Doxorubicinol; Doxorubicin; Aglycone metabolite; Pentose phosphate pathway; [13C]-NMR spectroscopy; Hemoglobin. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001200005 |
| |
|
|
|
