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| Merino-Contreras,María L.; Sánchez-Morales,Froylán; Jiménez-Badillo,María L.; Peña-Marín,Emyr S.; Álvarez-González,Carlos A.. |
| ABSTRACT Digestive proteases were partially characterized in sheepshead juveniles, using biochemical and electrophoretic techniques. Results showed higher activity level of the stomach proteases (2.39 ± 0.02 U mg protein-1) compared to the intestinal proteases (1.6 ± 0.1 U mg protein-1). The activity of trypsin, chymotrypsin, leucine aminopeptidase and carboxypeptidase A was also recorded. The optimum temperature of the stomach proteases was recorded at 45 °C, while for intestinal proteases was recorded at 55 °C. Stomach proteases showed less stability to temperature changes than intestinal proteases. An optimum pH of 2 was recorded for stomach proteases with high stability under acidic conditions, while an optimum pH of 9 was recorded for intestinal... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aquaculture; Inhibitors; Pepsin; Proteases; Zymogram. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252018000400206 |
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| Almeida,Ana Paula Gottlieb; Zardo,Everton Luis; Toni,Candida; Behr,Everton Rodolfo; Silva,Leila Picolli da; Vieira,João Paes; Loro,Vania Lúcia; Baldisserotto,Bernardo. |
| ABSTRACT The aim of this study was to determine the composition of gastrointestinal content and protease and lipase activities in summer and winter as well as to evaluate the relationship between digestive enzyme activity and centesimal composition of gastrointestinal content and feeding habits of two omnivorous species, Rhamdia quelen (Quoy & Gaimard, 1824) and Pimelodus maculatus (LaCèpede, 1803), and of two detritivorous species, Loricariichthys anus (Valenciennes, 1835) and Hypostomus commersoni (Valenciennes, 1836). The activities of pepsin, trypsin, chymotrypsin, and lipase, and the levels of proteins and lipids in the gastrointestinal tract, were evaluated. The enzyme activities were not related to the centesimal composition of gastrointestinal... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chymotrypsin; Fish; Lipase; Pepsin; Trypsin. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702018000100322 |
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| Vidal,Alessandra Roseline; Cechin,Carine da Fonseca; Cansian,Rogério Luis; Mello,Renius de Oliveira; Schmidt,Michele Mantelli; Demiate,Ivo Mottin; Kempka,Aniela Pinto; Dornelles,Rosa Cristina Prestes. |
| ABSTRACT: Enzymatic hydrolysis (pepsin) assisted with or without ultrasound in the functional properties of hydrolyzates from different collagens were analyzed. Degree of hydrolysis, antioxidant activity (DPPH) and antimicrobial activity (MIC) were assessed. The treatment that resulted in greater antioxidant activity for the fiber sample was with the use of 4% of enzyme and concomitant ultrasound (40.7%), leading to a degree of hydrolysis of 21.7%. For the powdered fiber sample the hydrolysis treatment with use of 4% of enzyme resulted in lower protein content (6.97mg/mL), higher degree of hydrolysis (19.9%) and greater antioxidant activity (38.6%). The hydrolyzates showed inhibitory capacity against gram-negative bacteria Salmonella choleraesuis and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pepsin; Degree of hydrolysis; Antioxidant activity; Antimicrobial activity; Ultrasound. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000300752 |
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| Michelotti,Bruna Tomazetti; Mori,Natacha Cossettin; Magnotti,Caio Cesar Franca; Heinzmann,Berta Maria; Almeida,Ana Paula Gottlieb; Cerqueira,Vinicius Ronzani; Baldisserotto,Bernardo. |
| ABSTRACT: Essential oils of plants whose main compound is citral showed beneficial effects when added to fish feed. The objective of the present study was to evaluate the dietary effect of the addition of citral on zootechnical parameters and digestive enzyme activities of Centropomus undecimalis. Juveniles were fed for 45 days with diets containing different amounts of citral (0.0 - control, 0.5, 1.0, and 2.0 mL per kg of diet). The water quality parameters were kept stable during the experiment and no mortality was observed. At the end of the experimental period, the treatment 0.5 mL citral per kg of diet had the lowest weight gain and specific growth rate, and the highest feed conversion, while the same parameters did not differ between the other... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Centropomus undecimalis; Amylase; Pepsin; Lipase; Growth; Marine fish farming. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000400651 |
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