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| Registros recuperados: 32 | |
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| Silva,L.F.; Pinto,P.S.A.; Ducas,C.T.S.; Santos,T.O.; Nieto,E.C.A.; Peixoto,R.P.M.G.. |
| Given the limited knowledge about the diagnosis of bovine cysticercosis by immunoblot, the aim of this study was to assess the applicability of this test, identifying key peptides with diagnostic value. Immunoblot assays were performed using total larval antigen of Taenia crassiceps and 60 sera of positive bovines for cysticercosis (30 naturally and 30 experimentally infected with T. saginata eggs), 30 sera of negative bovines for cysticercosis and 30 sera of bovines with other diseases (fascioliasis, hydatidosis and tuberculosis). The peptides of greater diagnostic importance, in descending order of accuracy (%), were as follows: 6-8kDa (90.8%), 129-143kDa (74.2%), 99-105kDa (71.7%) and 14-19kDa (71.1%). Cross-reactions, due to fascioliasis and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine cysticercosis; Diagnosis; Immunoblot; Taenia crassiceps; Peptides. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000300891 |
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| WILHELM,VIVIAN; VILLEGAS,JAIME; MIQUEL,ÁLVARO; ENGEL,ESTEBAN; BERNALES,SEBASTIÁN; VALENZUELA,PABLO D.T.; BURZIO,LUIS O.. |
| The complete sequence of the mitochondrial genome of Chinook salmon, Oncorhynchus tshawytscha, has been determined. The circular genome consisting of 16,644 base pairs encodes thirteen proteins, the 12S and 16S ribosomal RNAs, and 22 transfer RNAs. These genes are ordered in the same way as most other vertebrates. The nucleotide and amino acid sequences of the ribosomal RNAs and the thirteen protein-coding genes were compared with those of other salmonids such as Oncorhynchus mykiss, Salmo salar, Salvelinus fontinalis, Salvelinus alpinus and Coregonus lavaretus. The sequence features of the control region (D-loop), the origin of L-strand replication and a putative peptide codified by the 16S mitochondrial RNA are described and discussed. |
| Tipo: Journal article |
Palavras-chave: Salmonids; Mitochondria; Genome; Peptides; 16S rRNA. |
| Ano: 2003 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000200012 |
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| Figueiredo,Vitória Ribeiro Garcia de; Justus,Ariana; Pereira,Dafne Garcia; Georgetti,Sandra Regina; Ida,Elza Iouko; Kurozawa,Louise Emy. |
| Abstract This work aimed to evaluate the enzymatic hydrolysis of okara protein concentrate with respect to degree of hydrolysis (DH) in order to obtain a protein hydrolysate with high antioxidant capacity and aglycones isoflavone content. A central composite rotatable design was carried out to evaluate the influence of temperature (40 to 70°C), enzyme:substrate ratio (0.5 to 5.0%, g/100g protein) and pH (7.0 to 9.0) on DH. The optimal condition was 55°C, pH 9 and enzyme:substrate ratio of 5.0%, resulting a DH value of 35.5%. After protein hydrolysis at optimal condition, the antioxidant capacities of hydrolysate increased from 58.29 to 383.49 μM Trolox equivalent/g solids (ABTS method) and 2.41 to 15.32 μM Trolox equivalent/g solids (FRAP method) when... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Soy pulp; Peptides; Cleavage of protein; Isoflavone profile; Electrophoresis; Experimental design. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100505 |
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| Guzmán,Fanny; Barberis,Sonia; Illanes,Andrés. |
| Peptides are molecules of paramount importance in the fields of health care and nutrition. Several technologies for their production are now available, among which chemical and enzymatic synthesis are especially relevant. The present review pretends to establish a non-biased appreciation of the advantages, potentials, drawbacks and limitations of both technologies. Chemical synthesis is thoroughly reviewed and their potentials and limitations assessed, focusing on the different strategies and challenges for large-scale synthesis. Then, the enzymatic synthesis of peptides with proteolytic enzymes is reviewed considering medium, biocatalyst and substrate engineering, and recent advances and challenges in the field are analyzed. Even though chemical synthesis... |
| Tipo: Journal article |
Palavras-chave: Enzymatic synthesis; Peptides; Proteases; Solid-phase synthesis. |
| Ano: 2007 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200012 |
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| Mitta, Guillaume. |
| Les mécanismes immunitaires innés des vertébrés sont une mosaïque de mécanismes ancestraux partagés avec des animaux évolutivement plus primitifs. Les peptides antimicrobiens sont une de ces composantes conservées au travers du règne animal. En effet, ils participent vraisemblablement chez tous les métazoaires aux processi d'immunité innée. Toutefois, leurs implications dans la réponse anti-infectieuse peuvent être assez différentes. L'objet de cette thèse était de comprendre comment ces peptides participaient aux processi de défense de la moule. Une première phase de caractérisation biochimique de telles molécules avait été entreprise chez la moule, Mytilus galloprovincialis, dans le cadre de la thèse de Florence Hubert. Notre travail a consisté (i) à... |
| Tipo: Text |
Palavras-chave: Immunité; Peptides; Antibiotiques; Moule; Défensine. |
| Ano: 1999 |
URL: http://archimer.ifremer.fr/doc/00439/55101/56547.pdf |
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| Bernay, Benoît. |
| Despite the fact that the involvement of environmental factors in the physiology of the japanese oyster C. gigas is fully described, regulatory peptides are not known. In this study, we focused on peptidergic control of egg-laying. In order to characterize peptides involved in the regulation of egg-laying in C. gigas, the cuttlefish S. officinalis was used as a model. Firstly, regulatory peptides were investigated in the cuttlefish by peptidomic analysis. This approach led to the identification of eleven new ovarian regulatory peptides. Based on conserved tags, these peptides have been classified into two families: the SepCRPs (Sepia Capsule Releasing Peptides) and the OJPs (Ovarian Jelly Peptides). Released by oocytes into the genital coelom, they are... |
| Tipo: Text |
Palavras-chave: Ostréiculture; Ovaire; Spectrométrie de masse; Peptides; Céphalopodes; Bivalves; Mollusques. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/these-2566.pdf |
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| Cahu, Chantal; Zambonino, Jose-luis. |
| The production of fish larvae by hatchery shows an intensive increase for several species, but the specific nutritional requirements of larvae were poorly known until some years. We have conducted several experiments aimed to understand the specificities in larvae digestion, and to formulate a compound diet taking into account the differences compared to juveniles. The chosen species was the European sea bass, Dicentrarchus labrax, and the data were validated on different species. Larvae were fed live preys or experimental compound diet, especially formulated for the experiment. It appeared that pancreatic digestive enzymes, such as trypsin and amylase could be assayed in early ontogenesis, before mouth opening, and increase dramatically during the first... |
| Tipo: Text |
Palavras-chave: Peptides; Phospholipid; Digestive enzymes; Nutrition; Larvae; Dicentrarchus labrax. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3332.pdf |
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| Holford, Mande; Puillandre, Nicolas; Terryn, Yves; Cruaud, Corinne; Olivera, Baldomero; Bouchet, Philippe. |
| Toxoglossate marine gastropods, traditionally assigned to the families Conidae, Terebridae, and Turridae, are one of the most populous animal groups that use venom to capture their prey. These marine animals are generally characterized by a venom apparatus that consists of a muscular venom bulb and a tubular venom gland. The toxoglossan radula, often compared with a hypodermic needle for its use as a conduit to inject toxins into prey, is considered a major anatomical breakthrough that assisted in the successful initial radiation of these animals in the Cretaceous and early Tertiary. The pharmacological success of toxins from cone snails has made this group a star among biochemists and neuroscientists, but very little is known about toxins from the other... |
| Tipo: Text |
Palavras-chave: Life history similarities; Similar proboscis type; Morphology; Biology; Peptides; Radula. |
| Ano: 2009 |
URL: https://archimer.ifremer.fr/doc/00207/31788/30197.pdf |
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| Cahu, Chantal; Zambonino, Jose-luis; Takeuchi, Toshio. |
| Marine fish larvae undergo major functional and morphological changes during the developmental stages and several factors can interfere with the normal development of larvae and affect fry quality. Skeletal malformations, such as spinal malformation-scoliosis, lordosis, coiled vertebral column-, missing or additional fin rays, bending opercle or jaw malformations, are frequently observed in hatchery-reared larvae. This paper reviews the effects of some nutritional components on skeletal development in larvae of a number of fish species. In the dietary lipid fraction, for instance, it was proven that the phospholipid concentration affected the spinal malformation rate in sea bass fed a compound diet from mouth opening onwards. Phosphatidylinositol, in... |
| Tipo: Text |
Palavras-chave: Retinoic acid; Ascorbic acid; Amino acid; Phospholipid; Peptides; Skeletal development; Quality; Fish larvae. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/2003/publication-443.pdf |
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| Delcroix, Jeremy; Gatesoupe, F.j.; Desbruyeres, Elisabeth; Huelvan, Christine; Le Delliou, Herve; Le Gall, Marie-madeleine; Quazuguel, Patrick; Mazurais, David; Zambonino-infante, Jose-luis. |
| Protein hydrolysate is an essential component of dry starter diets for fish larvae, as promoting healthy development. Peptides are also suitable substrates for many intestinal microbes. Five experimental diets were compared to a control diet (CONT) supplemented with a commercial fish protein hydrolysate. Each diet contained one marine protein hydrolysate, which differed by the proportion of di- and tri-peptides, and by raw materials. Two diets (HYD4 and HYD5) stimulated larval growth compared with CONT. Two other diets (HYD1 and HYD2) yielded inferior growth. HYD1 was detrimental for survival, which was associated with an up-regulation of genes involved in inflammation and antioxidative responses, while the gene coding for osteocalcin was down-regulated in... |
| Tipo: Text |
Palavras-chave: Compound diets; Denaturing gradient gel electrophoresis; Digestive enzymes; Gene expression; Gut maturation; Peptides. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00197/30865/29268.pdf |
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| Registros recuperados: 32 | |
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