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| Carvalho,Vanda Marilza de; Marques,Rosimar Maria; Lapenta,Ana Sílvia; Machado,Maria de Fátima P.S.. |
| Polyacrylamide gel electrophoresis system (PAGE) and inhibition tests for biochemical characterization of alpha- and beta-esterases were used to obtain a functional classification of esterases fromAspidosperma polyneuron. The characterization of alpha- and beta-esterases from young leaves of A. polyneuron by the PAGE system showed fourteen esterase isozymes. The differential staining pattern showed that Est-2 isozyme hydrolyzes beta-naphthyl acetate; Est-6, Est-7 and Est-8 isozymes hydrolyze alpha-naphthyl acetate, and Est-1, Est-3, Est-4, Est-5, Est-9, Est-10, Est-11, Est-12, Est-13, and Est-14 isozymes hydrolyze both alpha- and b-naphthyl acetate. Inhibition pattern of a- and beta-esterases showed that Folidol is a more potent inhibitor that Malathion,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Esterase classification; Peroba-rosa; Ops; Inhibition test. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200013 |
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| Torezan,José Marcelo Domingues; Souza,Rogério Fernandes de; Ruas,Paulo Maurício; Ruas,Claudete de Fátima; Camargo,Elvis Hassmann; Vanzela,André Luís Laforga. |
| RAPD was used to access the genetic variability in Aspisdosperma polyneuron, a long-lived, late-reproducing tropical tree, and highly important for the Atlantic Forest. RAPD profiles from adults (pre-fragmentation, >300 years old) and seedlings (post-fragmentation, <<50 years old) were analyzed. Results showed a decrease of genetic polymorphism of post-fragmentation cohorts in small fragments and higher genetic diversity within population. The genetic diversity distribution suggested the establishment of fragments as protected reserves, and the transference of seedlings among fragments for conservation of A. polyneuron. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aspidosperma; DNA markers; Forest fragmentation; Genetic variability; Peroba-rosa; RAPD. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000200002 |
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