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Investigation of toxic factors affecting cells of rat brains exposed to 3-methylcatechol BABT
Barreto,George Emílio Sampaio; Oliveira,Elineusa Silva de; Villar,Flávio Augusto Guerreiro Aragão de; Santos,Gleide Souza dos; Lima,Rute Maria Ferreira; Costa,Silvia Lima; Costa,Maria de Fátima Dias; Egito,Eryvaldo Sócrates Tabosa; El-Bachá,Ramon Santos.
The aim of this work was to study the effects of 3MC on the peroxidation of biomolecules in nuclear fractions and nonsynaptic mitochondrial respiration in organelles obtained from rat brains. The cytotoxicity towards rat primary astrocytes in vitro was also tested. 3MC at 1mM oxidized consuming oxygen at a rate of 1.98 ± 0.19 µM.min-1 and formed reactive quinones. At the same concentration, 3MC induced peroxidation of biomolecules in nuclear fractions obtained from rat brain homogenates and inhibited state 2 FADH2-linked respiration in nonsynaptic mitochondria. Furthermore, 3MC oxidized in the culture medium, leading to the formation of quinones. This toluene metabolite was cytotoxic to rat primary astrocytes. The concentration that killed 50% of cells...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Astrocytes; Brain; Cytotoxicity; 3-methylcatechol; Peroxidation; Respiration.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000500012
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Inhibition of rat microsomal lipid peroxidation by the oral administration of D002 BJMBR
Menéndez,R.; Amor,A.M.; González,R.M.; Jiménez,S.; Más,R..
The effect of D002, a defined mixture of higher primary alcohols purified from bee wax, on in vivo and in vitro lipid peroxidation was studied. The extent of lipid peroxidation was measured on the basis of the levels of thiobarbituric acid reactive substances (TBARS). When D002 (5-100 mg/kg body weight) was administered orally to rats for two weeks, a partial inhibition of the in vitro enzymatic and non-enzymatic lipid peroxidation was observed in liver and brain microsomes. Maximal protection (46%) occurred at a dose of 25 mg/kg. D002 behaved differently depending on both the presence of NADPH and the integrity of liver microsomes, which suggests that under conditions where microsomal metabolism was favored the protective effect of D002 was increased....
Tipo: Info:eu-repo/semantics/article Palavras-chave: D002; Higher aliphatic alcohols; Microsomes; Lipids; Peroxidation.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000100012
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Determination of serum aluminum, platelet aggregation and lipid peroxidation in hemodialyzed patients BJMBR
Neiva,T.J.C.; Benedetti,A.L.; Tanaka,S.M.C.N.; Santos,J.I.; D'Amico,E.A..
Aluminum (Al3+) overload is frequently associated with lipid peroxidation and neurological disorders. Aluminum accumulation is also reported to be related to renal impairment, anemia and other clinical complications in hemodialysis patients. The aim of the present study was to determine the degree of lipid peroxidation, platelet aggregation and serum aluminum in patients receiving regular hemodialytic treatment. The level of plasma lipid peroxidation was evaluated on the basis of thiobarbituric acid reactive substances (TBARS). Mean platelet peroxidation in patients undergoing hemodialysis was significantly higher than in normal controls (2.7 ± 0.03 vs 1.8 ± 0.06 nmol/l, P<0.05). Platelet aggregation and serum aluminum levels were determined by a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aluminum; Peroxidation; Hemodialysis; Platelets.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000300009
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The sensitivity of sunflower (Helianthus annuus L.) plants to UV-B radiation is altered by nitrogen status Ciência Rural
Cechin,Inês; Gonzalez,Gisely Cristina; Corniani,Natália; Fumis,Terezinha de Fátima.
ABSTRACT: Interaction effects between nitrogen and UV-B radiation were studied in sunflower (Helianthus annuus L. variety IAC-Iarama) plants grown in a greenhouse under natural photoperiod conditions. Plants were irradiated with 0.8W m-2 (control) or 8.0W m-2 (+UV-B) of UV-B radiation for 7h per day. The plants were grown in pots containing vermiculite and watered with 70% of full strength nitrogen-free Long Ashton solution, containing either low (42.3ppm) or high (282ppm) nitrogen as ammonium nitrate. High nitrogen increased dry matter of stem, leaves and shoot, photosynthetic pigments and photosynthesis (A) without any alteration in stomatal conductance (gs) nor transpiration (E) while it reduced the intercellular CO2 (Ci) concentration, and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidants; Growth; Malondialdehyde; Peroxidation; Photosynthesis.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000200251
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