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| TORO,ANDRÉS; ARREDONDO,CRISTIAN; CORDOVA,GONZALO; ARAYA,CLAUDIA; PALACIOS,JOSÉ L; VENEGAS,ALEJANDRO; MORITA,MASASHI; IMANAKA,TSUNEO; SANTOS,MANUEL J. |
| Peroxisomes are thought to be formed by division of pre-existing peroxisomes after the import of newly synthesized proteins. However, it has been recently suggested that the endoplasmic reticulum (ER) provides an alternative de novo mechanism for peroxisome biogenesis in some cells. To test a possible role of the ER-Golgi transit in peroxisome biogenesis in mammalian cells, we evaluated the biogenesis of three peroxisomal membrane proteins (PMPs): ALDRP (adrenoleukodystrophy related protein), PMP70 and Pex3p in CHO cells. We constructed chimeric genes encoding these PMPs and green fluorescent protein (GFP), and transiently transfected them to wild type and mutant CHO cells, in which normal peroxisomes were replaced by peroxisomal membrane ghosts. The... |
| Tipo: Journal article |
Palavras-chave: Adrenoleukodystrophy; ALDRP; Endoplasmic reticulum; Peroxisome biogenesis; Pex3p and PMP70. |
| Ano: 2007 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000200014 |
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