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Koga,Paula Célia Mariko; Menezes,Caroline Anunciação; Lima,Flávia Afonso; Nara,Júlia Mitico; Magalhães,Caroline Arantes; Cianciarullo,Aurora Marques; Ferreira-Júnior,Jorge Mario da Costa; Trabulsi,Luiz Rachid; Mendes-Ledesma,Meire Roberta Bresciani; Piazza,Roxane Maria Fontes. |
Intimins are outer membrane proteins expressed by enteric bacterial pathogens capable of inducing intestinal attachment-and-effacement lesion (A/E). Through immunoblotting, immunofluorescence, flow citometry and immunogold we observed that the obtained polyclonal antibody against conserved intimin region recognizes the different intimin subtypes and suggests that it can be used as a tool for EPEC and EHEC detection. Besides, immuno-dot assay seems to be a possible alternative as a capture method. |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Intimin; Phenotypical analysis; Polyclonal antibody; EPEC; EHEC. |
Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500002 |
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