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| Li,Jingjing; Xiong,Ying; Sun,Shimin; Yu,Lehan; Huang,Chunhong. |
| Abstract Background: The gamma-type phospholipase A2 inhibitor (ΡLIγ) is a natural protein commonly found in snake serum, which can neutralize pathophysiological effects of snake venom phospholipases A2. Therefore, this protein is a potential candidate to the development of a novel antivenom. To the best of our knowledge, there is no antibody currently available for PLIγ identification and characterization. Methods: Bioinformatics prediction of epitope using DNAStar software was performed based on the sequence of Sinonatrix annularis PLIγ (SaPLIγ). The best epitope 151CPVLRLSNRTHEANRNDLIKVA172 was chosen and synthesized, and then conjugated to keyhole limpet hemocyanin and bovine serum albumin for use as an immunogen and plate-coating antigen,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Monoclonal antibody; Phospholipase A2 inhibitor; Epitope prediction. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100314 |
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| Fortes-Dias,Consuelo L.; Macedo,Diego Henrique Fagundes; Barbosa,Rafaella Pereira; Souza-Silva,Gabriel; Ortolani,Paula Ladeira. |
| Abstract Background: Endogenous phospholipase A2 inhibitors from snake blood (sbPLIs) have been isolated from several species around the world, with the primary function of self-protection against the action of toxic phospholipases A2. In American snakes, sbPLIs were solely described in pit vipers, in which the natural protection role is justified. In this study, we described a sbPLI in Boa constrictor (popularly known as jiboia), a non-venomous snake species from America. Methods: PLA2 inhibitory activity was tested in the blood plasma of B. constrictor using C. d. terrificus venom as the enzyme source. Antibodies developed against CNF, a sbγPLI from Crotalus durissus terrificus, were used to investigate the presence of homologues in the blood plasma... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Phospholipase A2 inhibitor; Phospholipase A2; Boidae; Snakes; Rattlesnake venom. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992020000100305 |
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