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| Yamamoto, Keisuke; Takada, Yuichi; Kida, Akemi; Akematsu, Takahiko; Kotsuki, Hiyoshizo; Matsuoka, Tatsuomi. |
| In the absence of O2, the step-up photophobic response of Blepharisma japonicum is suppressed. To evaluate the possibility that reactivated oxygen species (ROS) produced by blepharismin photosensitization are involved in the primary transduction for the step-up photophobic response of B. japonicum, the effects of OH radical scavengers and the singlet oxygen quencher on the photophobic response were examined. The reagents used in the present assays hardly suppressed the step-up photophobic response, even though OH radical scavengers were rescued from the photodynamic killing of the cells. Alternatively, in the presence of an electron acceptor, the latency of the step-up photophobic response was prolonged. In addition, oxyblepharismin fluorescence was... |
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Palavras-chave: Blepharisma; Phototransduction; Reactivated oxygen species. |
| Ano: 2007 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1049 |
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| Muñoz,Yorka; Fuenzalida,Karen; Bronfman,Miguel; Gatica,Arnaldo; Sepúlveda,Marcelo; Bacigalupo,Juan; Roth,Alejandro D; Delgado,Ricardo. |
| Phototransduction, the mechanism underlying the electrical response to light in photoreceptor cells, has been thoroughly investigated in Drosophila melanogaster, an essential model in signal transduction research. These cells present a highly specialized photosensitive membrane consisting of thousands of microvilli forming a prominent structure termed a rhabdomere. These microvilli encompass the phototransduction proteins, most of which are transmembrane and exclusively rhabdomeric. Rhabdomere membrane lipids play a crucial role in the activation of the transient receptor potential ionic channels (TRP and TRPL) responsible for initiating the photoresponse. Despite its importance, rhabdomere lipid composition has not been established. We developed a novel... |
| Tipo: Journal article |
Palavras-chave: Rhabdomere; Drosophila; Microvilli; Phototransduction; TRP channel; Fatty acid analysis. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000300010 |
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