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Walter,Alfredo; Carvalho,Júlio Cesar de; Soccol,Vanete Thomaz; Faria,Ana Bárbara Bisinella de; Ghiggi,Vanessa; Soccol,Carlos Ricardo. |
The aim of this work was to investigate the production of phycocyanin by Spirulina platensis under different spectra of light. The dependent variables evaluated were the amount of phycocyanin obtained and its purity, demonstrating that there might be a restructuring of phycobilisomes, especially when the culture was subjected to red light, which increased the purity level up to 33% with a reduction of 16% in phycocyanin content, but with higher photosynthetic efficiency compared to natural light. |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Spirulina; Phycocyanin; Phycobilisome; Pigment; Spectrum; Chromatic acclimation. |
Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400005 |
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Pádula,M.; Boiteux,S.. |
In the present study, we analyzed DNA damage induced by phycocyanin (PHY) in the presence of visible light (VL) using a set of repair endonucleases purified from Escherichia coli. We demonstrated that the profile of DNA damage induced by PHY is clearly different from that induced by molecules that exert deleterious effects on DNA involving solely singlet oxygen as reactive species. Most of PHY-induced lesions are single strand breaks and, to a lesser extent, base oxidized sites, which are recognized by Nth, Nfo and Fpg enzymes. High pressure liquid chromatography coupled to electrochemical detection revealed that PHY photosensitization did not induce 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) at detectable levels. DNA repair after PHY... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Phycocyanin; Saccharomyces cerevisiae; OGG1; Nucleotide excision repair. |
Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000900002 |
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Rizzo,Roberta Ferreira; Santos,Beatriz do Nascimento Corrêia dos; Castro,Gabriela Fernandes Pepe da Silva de; Passos,Thaís Souza; Nascimento,Manuela de Abreu; Guerra,Hevelyn Dantas; Silva,Carla Guidone da; Dias,Daiana da Silva; Domingues,Josiane Roberto; Lima-Araújo,Kátia Gomes de. |
There has been an increase in investment in research on new sources of natural pigments for food application. Some cyanobacteria can change the structures responsible for light harvesting and cellular processes according to the wavelength and light intensity. This phenomenon has been described as complementary chromatic adaptation. The present study aimed to investigate the growth of Arthrospira platensis using different light qualities, irradiance, and wavelength by evaluating the production of biomass, proteins, and phycobiliproteins. The occurrence of the chromatic adaptation phenomenon in this cyanobacterium was also investigated. The microorganism used in this study, A. platensis, was grown in a Zarrouk medium under three irradiance levels, 50, 100,... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Natural pigment; Phycobiliproteins; Phycocyanin; Arthrospira platensis. |
Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612015000200247 |
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Juin, Camille; Cherouvrier, Jean-rene; Thiery, Valerie; Gagez, Anne-laure; Berard, Jean-baptiste; Joguet, Nicolas; Kaas, Raymond; Cadoret, Jean-paul; Picot, Laurent. |
In the present study, microwave-assisted extraction was first employed to extract the phycobiliproteins of Porphyridium purpureum (Pp). Freeze-dried Pp cells were subjected to microwave-assisted extraction (MAE) to extract phycoerythin (PE), phycocyanin (PC), and allophycocyanin (APC). MAE combined reproducibility and high extraction yields and allowed a 180- to 1,080-fold reduction of the extraction time compared to a conventional soaking process. The maximal PE extraction yield was obtained after 10-s MAE at 40 A degrees C, and PE was thermally damaged at temperatures higher than 40 A degrees C. In contrast, a flash irradiation for 10 s at 100 A degrees C was the best process to efficiently extract PC and APC, as it combined a high temperature necessary... |
Tipo: Text |
Palavras-chave: Allophycocyanin; MAE; Microalgae; Microwave; Phycobiliprotein; Phycocyanin; Phycoerythrin; Porphyridium; Thylakoid. |
Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00253/36439/35169.pdf |
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