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Restriction fragment analysis of the ribosomal DNA of Paratelmatobius and Scythrophrys species (Anura, Leptodactylidae) Genet. Mol. Biol.
Lourenço,Luciana B.; Garcia,Paulo C.A.; Recco-Pimentel,Shirlei M..
Physical maps of the ribosomal RNA gene 28S of species belonging to the genera Paratelmatobius and Scythrophrys were constructed, using five restriction endonucleases. The restriction sites for Bam HI, Bgl II, Bst EII, and Eco RI had similar positions in all species, although there were interspecific differences in the size of the restriction fragments obtained. An additional Pvu II site was found in Scythrophrys specimens from Piraquara (State of Paraná, Brazil) and from São Bento do Sul (State of Santa Catarina, Brazil), but not in the Scythrophrys specimens from Rancho Queimado (State of Santa Catarina, Brazil). This finding is in agreement with the hypothesis regarding the existence of two species in the genus Scythrophrys. On the other hand, the extra...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ribosomal DNA; 28S rDNA gene; Restriction sites; Physical map; Paratelmatobius; Scythrophrys; Leptodactylidae; Anura.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200006
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แผนที่กายภาพพันธุกรรมที่ควบคุมขนาดของรากและการค้นหายีนที่เกี่ยวข้องกับความสามารถชอนไชของรากข้าวบนโครโมโซมที่ 4 Thai Agricultural
Varapong Chamarerk.
Major quantitative trait loci (QTL) associated with basal root thickness (BRT) on chromosome 4 was used as the target for physical mapping. Two parental rice lines contrasting in their root characteristics, CT9993-5-10-1-M (CT) and IR62266-42-6-2 (IR), were used in this study. The objectives of this study were to fine map the major BRT QTL on chromosome 4 in rice and to isolate candidate genes related to root penetration index (RPI) trait which located in this region. The BAC library available for screening in this study was constructed from a rice cultivar Nipponbare (japonica) with average insert sizes of 130 to 150 kb and with a 10X genome equivalent. The RFLP markers located near the target BRT QTL on rice chromosome 4 were used for screening BAC...
Tipo: Collection Palavras-chave: Oryza sativa (japonica); Physical map; Quantitative trait loci (QTL); Basal root thickness (BRT); Root penetration index (RPI); Differential display (DD) technique; Chromosome 4; ข้าวจาปอนิกา; แผนที่กายภาพ; ลักษณะทางพันธุกรรมเชิงปริมาณ; ขนาดของรากข้าว; การชอนไชของรากข้าว; เทคนิค DD (Differential Display); โครโมโซมที่ 4; โมเลกุลเครื่องหมาย; ดีเอ็นเอ.
Ano: 2008 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5103
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