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	Provedor de dados Biocell (1) Genet. Mol. Biol. (1) Autor Affara,Nabeel (1) Brenig,Bertram (1) Chen,Congying (1) Greizerstein,Eduardo J. (1) Heinz,Ruth A. (1) Hopp,H. Esteban (1) Huang,Lusheng (1) Paniego,Norma (1) Poggio,Lidia (1) Quilter,Claire (1) Ren,Jun (1) Sargent,Carole (1) Talia,Paola (1) Yang,Zhuqing (1) Mais... Palavra-chave Physical mapping (2) BAC-FISH (1) C-PRINS (1) Expression profile (1) Molecular characterization (1) Single-copy sequences (1) Sunflower (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2011 (1) 2010 (1) País Argentina (1) Brazil (1) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Cloning, mapping and molecular characterization of porcine progesterone receptor membrane component 2 (PGRMC2) gene Genet. Mol. Biol. Chen,Congying; Sargent,Carole; Quilter,Claire; Yang,Zhuqing; Ren,Jun; Affara,Nabeel; Brenig,Bertram; Huang,Lusheng. Progesterone plays an important role in sow reproduction by stimulating classic genomic pathways via nuclear receptors and non-genomic pathways via membrane receptors such a progesterone receptor membrane component 2 (PGRMC2). In this work, we used radiation hybrid mapping to assign PGRMC2 to pig chromosome 8 and observed that this receptor has two transcripts in pigs. The full-length cDNA of the large transcript is 1858 bp long and contains a 669-bp open reading frame (ORF) encoding a protein of 223 amino acids. The shorter transcript encodes a protein of 170 amino acids. The porcine PGRMC2 gene consists of three exons 446 bp, 156 bp and 1259 bp in length. The promoter sequence is GC-rich and lacks a typical TATA box. Several putative cis-regulatory DNA... Tipo: Info:eu-repo/semantics/article Palavras-chave: Expression profile; Molecular characterization; Physical mapping. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300015 Detection of single copy sequences using BAC-FISH and C-PRINS techniques in sunflower chromosomes Biocell Talia,Paola; Greizerstein,Eduardo J.; Hopp,H. Esteban; Paniego,Norma; Poggio,Lidia; Heinz,Ruth A.. Bacterial artificial chromosome - fluorescence in situ hybridization (BAC-FISH) and cycling-primed in situ labeling (C-PRINS) techniques were evaluated for integration of physical and genetic maps of sunflower (Helianthus annuus L.). Single-site SSR markers were selected from three linkage groups of a high-density sunflower genetic map. This selection was based on previously identified QTL associated to S. sclerotiorum. These markers were used to select BACs contaning single copy sequences for BAC-FISH aplication. Blocking of highly dispersed repetitive sunflower sequences reduced unspecific hybridization, and allowed the detection of specific signals for BACs containing SSR markers HA4222 and HA2600, anchored to LG 16 and LG 10, respectively. Single-site... Tipo: Info:eu-repo/semantics/article Palavras-chave: Sunflower; BAC-FISH; C-PRINS; Single-copy sequences; Physical mapping. Ano: 2011 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452011000100003 Registros recuperados: 2 Primeira ... 1 ... Última

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