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Extraction and anticoagulant activity of sulfated polysaccharides from Caulerpa cupressoides var. lycopodium (Vahl) C. Agardh (Chlorophyceae) - doi: 10.4025/actascibiolsci.v33i2.6243 Biological Sciences
Rodrigues, José Ariévilo Gurgel; REDE NORDESTE DE BIOTECNOLOGIA; Vanderlei, Edfranck de Sousa Oliveira; UNIVERSIDADE FEDERAL DO CEARÁ; Quinderé, Ana Luíza Gomes; UNIVERSIDADE FEDERAL DO CEARÁ; Fontes, Bruno Pedrosa; UNIVERSIDADE FEDERAL DO CEARÁ; Queiroz, Ismael Nilo Lino de; UNIVERSIDADE FEDERAL DO CEARÁ; Benevides, Norma Maria Barros; UNIVERSIDADE FEDERAL DO CEARÁ.
The reportedly low standard quality of heparin (HEP) for use in cardiac surgeries has led to concern in the Brazilian and international markets. Sulfated polysaccharides (SPs) from seaweeds have been regarded as promising substitutes for HEP. The aim of this study was to sequentially extract total SPs (TSPs) from Caulerpa cupressoides (Chlorophyceae) with papain in 100 mM sodium acetate buffer (pH 5.0) containing 5 mM cysteine and 5 mM EDTA, followed by fractionation by ion-exchange chromatography (DEAE-cellulose), and then evaluate the anticoagulant potential of SP fractions by activated partial thromboplastin time (APTT) using normal human plasma and compare it to standard HEP (193 IU mg-1). The obtained fractions were chemically characterized by...
Tipo: Bioprospecção Palavras-chave: 2.08.01.00-9 Chlorophyta; Sequential extractions; Physical-chemical characterization; Coagulation test 2.08.01.00-9 Chlorophyta; Sequential extractions; Physical-chemical characterization; Coagulation test.
Ano: 2011 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/6243
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Parboiled rice: chemical composition and the occurrence of mycotoxins Ciênc. Tecnol. Aliment.
Dors,Giniani Carla; Bierhals,Vânia da Silva; Badiale-Furlong,Eliana.
The objective of this work was to evaluate the incidence of aflatoxin B1 (AFB B1), deoxynivalenol (DON), ochratoxin A (OTA), and zearalenone (ZEA) in parboiled rice with respect to its chemical composition. Eight lots from five different brands of parboiled rice were collected in four samplings, at different seasons, until the amount of 32 lots. It was observed that: DON was present in 22% of the samples (from 180 to 400 ppb); ZEA in 19% (from 317 to 396 ppb); OTA in 12.5% (from 13 and 26 ppb); and AFB B1 in 9% (from 11 to 74 ppb). The results of the chemical composition were not different from those previously mentioned in the literature concerning parboiled rice. The ash and phenol levels in the contaminated parboiled rice samples suggested that those...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Deoxynivalenol; Ochratoxin; Physical-chemical characterization; Zearalenone.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612011000100025
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