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| Shao,Huanhuan; Yong,Bin; Xu,Pan; Zheng,Haiyan; Liao,Ruoxing; Wang,Xiaoyan; Li,Xinyu; Zhang,Li; Shen,Jiabin. |
| ABSTRACT Phytoene synthase (PSY) is the rate-limiting enzyme for carotenoid biosynthesis. To date, several studies focused on PSY genes in the context of abiotic stress responses. In this study, two phytoene synthase encoding genes, IbPSY1 and IbPSY2, were identified from a published transcriptome and bioinformatic analysis showed that they shared conserved domains with phytoene synthases from other plants. The IbPSY1 gene was cloned and carefully characterized. Digital gene expression profiling (DGE) showed that the highest transcription level of IbPSY1 was in young leaves, and the lowest level was in stems. In vivo expression levels of IbPSY1 under abiotic stress were observed to be highest in stems at day 11. Over-expression of IbPSY1 in Escherichia... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ipomoea batatas; Phytoene synthase; Abiotic stress. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100422 |
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