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Simplified Transformation of Ostreococcus tauri Using Polyethylene Glycol ArchiMer
Sanchez, Frédéric; Geffroy, Solene; Norest, Manon; Yau, Sheree; Moreau, Hervé; Grimsley, Nigel.
Ostreococcus tauri is an easily cultured representative of unicellular algae (class Mamiellophyceae) that abound in oceans worldwide. Eight complete 13–22 Mb genomes of phylogenetically divergent species within this class are available, and their DNA sequences are nearly always present in metagenomic data produced from marine samples. Here we describe a simplified and robust transformation protocol for the smallest of these algae (O. tauri). Polyethylene glycol (PEG) treatment was much more efficient than the previously described electroporation protocol. Short (2 min or less) incubation times in PEG gave >104 transformants per microgram DNA. The time of cell recovery after transformation could be reduced to a few hours, permitting the experiment to be...
Tipo: Text Palavras-chave: Chlorophyta; Prasinophyte; Mamiellophyceae; Ostreococcus; Plankton; Picoeukaryote; Ecosystem; Bioluminescence; Promoter; Luciferase; Gene expression.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00500/61170/64639.pdf
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Phytoplankton seasonal dynamics in a Mediterranean coastal lagoon: emphasis on the picoeukaryote community ArchiMer
Bec, Béatrice; Husseini Ratrema, Julie; Collos, Yves; Souchu, Philippe; Vaquer, André.
The dynamics of the phytoplankton community were investigated in a marine coastal lagoon (Thau, NW Mediterranean) from February 1999 to January 2000. Dilution experiments, chlorophyll a (Chl a) size-fractionation and primary production measurements were conducted monthly. Maximum growth and microzooplankton grazing rates were estimated from Chl a biomass fractions to separate pico- from nano- and microphytoplankton and by flow cytometry to distinguish between picoeukaryotes and picocyanobacteria. In spring, the phytoplankton community was dominated by Chaetoceros sp. and Skeletonema costatum, which represented most of biomass (B) and primary production (P). Nano- and microphytoplankton growth was controlled by nutrient availability and exceeded losses due...
Tipo: Text Palavras-chave: Coastal lagoon; NW Mediterranean; Picoeukaryote; Phytoplankton.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-726.pdf
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