|
|
|
| Registros recuperados: 64 | |
|
|
| Cordeiro,Alexandra F. da S.; Nääs,Irenilza de A.; Oliveira,Stanley R. de M.; Violaro,Fabio; Almeida,Andréia C. M. de. |
| Among the challenges of pig farming in today's competitive market, there is factor of the product traceability that ensures, among many points, animal welfare. Vocalization is a valuable tool to identify situations of stress in pigs, and it can be used in welfare records for traceability. The objective of this work was to identify stress in piglets using vocalization, calling this stress on three levels: no stress, moderate stress, and acute stress. An experiment was conducted on a commercial farm in the municipality of Holambra, São Paulo State , where vocalizations of twenty piglets were recorded during the castration procedure, and separated into two groups: without anesthesia and local anesthesia with lidocaine base. For the recording of acoustic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Vocal expression; Well-being; Level of pain; Pig. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69162012000200001 |
| |
|
|
| Gan,LiNa; Qin,WeiYun; Wu,Sen; Wu,ShengLong; Bao,WenBin. |
| Abstract MYD88 plays an important role in the immune response against infections. To analyze MYD88 gene expression during different stages of pig development, we used real-time PCR. MYD88 was seen expressed in all tissues examined. MYD88 expression in spleen, lungs, and thymus reached its highest value from 7 to 14 days of age and decreased thereafter. Expression in lymph nodes was high until 28 days of age and then it declined after weaning, with stable low levels in adult pigs. MYD88 expression was high before 35 days of age in the small intestine (duodenum, jejunum, and ileum), where it reached its highest value from 7 to 14 days of age. MYD88 expression in the small intestine declined post-weaning and remained relatively low during adulthood. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: MYD88; Pig; Developmental expression; Immune response. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000100119 |
| |
|
| |
|
|
| Ventura,Henrique T.; Lopes,Paulo S.; Peloso,José V.; Guimarães,Simone E.F.; Carneiro,Antonio Policarpo S.; Carneiro,Paulo L.S.. |
| The association between carcass and ham traits in a pig population used to produce dry-cured ham was studied using canonical correlation analysis. The carcass traits examined were hot carcass weight (HCW), backfat thickness (BT) and loin depth (LD), and the ham traits studied were gross ham weight (GHW), trimmed ham weight (THW), ham inner layer fat thickness (HIFT), ham outer layer fat thickness (HOFT), pH (pH) and the Göfo value. Carcass and ham traits are not independent. The canonical correlations (r) between the carcass and ham traits at 130 kg were 0.77, 0.24 and 0.20 for the first, second and third canonical pair, respectively, and were all significant (p < 0.01) by the Wilks test. The corresponding canonical correlations between the three... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Canonical correlation; Carcass; Ham; Pig. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000300014 |
| |
|
|
| Li,Xinyun; Liu,Huazhen; Shulin,Yang; Tang,Zhonglin; Ma,Yuehui; Chu,Mingxing; Li,Kui. |
| The myeloid differentiation primary response protein 88 (Myd88) is an essential adaptor protein, which mediates in all Toll-like receptor (TLR) members signal transduction, except for TLR3. In this study, the 4464 bp genomic sequence of porcine Myd88 was first isolated, whereupon tissue distribution, chromosome mapping and single nucleotide polymorphism (SNP) were analyzed. Our results revealed that porcine Myd88 gene, which was located at chromosome 13 linked with marker S0288 (distance = 40 cR; LOD = 8.66), was widely expressed in all the examined tissues. There were 16 potential SNPs in the isolated genome fragment. SNP 797T/C in the first intron was studied, with no significant association being found between the genotype and immune traits in pigs (p... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Myd88; TLR; Polymorphism; Pig; Chromosome mapping. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200015 |
| |
|
|
| Wang,Dongxu; Wang,Guodong; Yang,Hao; Liu,Haibo; Li,Cuie; Li,Xiaolan; Lin,Chao; Song,Yuning; Li,Zhanjun; Liu,Dianfeng. |
| Abstract The sexually dimorphic expression of H19/IGF2 is evolutionarily conserved. To investigate whether the expression of H19/IGF2 in the female porcine eye is sex-dependent, gene expression and methylation status were evaluated using quantitative real-time PCR (qPCR) and bisulfite sequencing PCR (BSP). We hypothesized that H19/IGF2 might exhibit a different DNA methylation status in the female eye. In order to evaluate our hypothesis, parthenogenetic (PA) cells were used for analysis by qPCR and BSP. Our results showed that H19 and IGF2 were over-expressed in the female eye compared with the male eye (3-fold and 2-fold, respectively). We observed a normal monoallelic methylation pattern for H19 differentially methylated regions (DMRs). Compared with... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: H19/IGF2; Gene expression; DNA methylation; Parthenogenetic; Pig. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100153 |
| |
|
|
| Franco,Maurício Machaim; Antunes,Robson Carlos; Oliveira,Katiana Mello de; Pereira,Cícero Donizete; Biase,Fernando Henrique; Nunes,Francis de Morais Franco; Goulart,Luiz Ricardo. |
| Fourty-six non-castrated, halothane-free, male Landrace pigs were genotyped by PCR-RFLP for the Rsa I polymorphism in the PIT1 gene and classified into AA and AB genotypes. Total RNA was extracted from the pituitaries and the relative quantities of growth hormone (GH) mRNA were determined by semi-quantitative RT-PCR. Pigs with the AB genotype had higher levels of GH mRNA than those with the AA genotype (p = 0.034; Kruskal-Wallis test). This result suggests that the Rsa I polymorphism may be involved in Pit-1 protein expression or function, which in turn may influence GH transcription and expression. Thus, the Rsa I PIT1 gene polymorphism in this pig line may be used as a molecular marker to identify higher GH expression and possibly select for carcass and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: PIT1 gene; Growth hormone mRNA; Semi-quantitative RT-PCR; Pig. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100003 |
| |
|
|
| Band,Guilherme de Oliveira; Guimarães,Simone Eliza Facioni; Lopes,Paulo Sávio; Schierholt,Alex Sandro; Silva,Kleibe Moraes; Pires,Aldrin Vieira; Benevenuto Júnior,Augusto Aloísio; Gomide,Lúcio Alberto de Miranda. |
| The PSS genotypes of 596 F2 pigs produced by initial mating of Brazilian commercial sows and native boars were characterized by PCR-RFLP and the pork quality traits were evaluated. Among the 596 pigs studied, 493 (82.7%) were NN and 103 (17.3%) were Nn. There were no differences between NN and Nn pigs in the following pork qualities: pHu (5.71 ± 0.16 vs 5.70 ± 0.11), intramuscular fat (1.55 ± 0.64% vs 1.65 ± 0.67%), shear force (5552 ± 878 g/1.2 cm vs 5507 ± 826 g/1.2 cm), lightness (44.96 ± 2.05 vs 45.01 ± 1.92), redness (0.64 ± 0.60 vs 0.79 ± 0.55), yellowness (6.62 ± 0.56 vs 6.65 ± 0.48), hue (84.28 ± 5.53 vs 83.41 ± 4.85), or chroma (6.68 ± 0.52 vs 6.73 ± 0.52). However, pork from Nn pigs had a significantly (p < 0.05) lower pH45 (6.41 ± 0.27 vs... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Meat quality; PCR-RFLP; Pork; PSE; PSS; Pig. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100015 |
| |
|
|
| Reis,Evelyze Pinheiro dos; Paixão,Débora Martins; Brustolini,Otávio José Bernardes; Silva,Fabyano Fonseca e; Silva,Walmir; Araújo,Flávio Marcos Gomes de; Salim,Anna Christina de Matos; Oliveira,Guilherme; Guimarães,Simone Eliza Facioni. |
| Abstract This study used qRT-PCR to examine variation in the expression of 13 myogenes during muscle development in four prenatal periods (21, 40, 70 and 90 days post-insemination) in commercial (the three-way Duroc, Landrace and Large-White cross) and local Piau pig breeds that differ in muscle mass. There was no variation in the expression of the CHD8, EID2B, HIF1AN, IKBKB, RSPO3, SOX7 and SUFU genes at the various prenatal ages or between breeds. The MAP2K1 and RBM24 genes showed similar expression between commercial and Piau pigs but greater expression (p < 0.05) in at least one prenatal period. Pair-wise comparisons of prenatal periods in each breed showed that only the CSRP3, LEF1, MRAS and MYOG genes had higher expression (p < 0.05) in at... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Commercial line; Gene expression; Myogenesis; Pig; Piau breed. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000400589 |
| |
|
|
| Yang,Seung Hak; Lim,Joung Soo; Khan,Modabber Ahmed; Kim,Bong Soo; Choi,Dong Yoon; Lee,Eun Young; Ahn,Hee Kwon. |
| The leachate generated by the decomposition of animal carcass has been implicated as an environmental contaminant surrounding the burial site. High-throughput nucleotide sequencing was conducted to investigate the bacterial communities in leachates from the decomposition of pig carcasses. We acquired 51,230 reads from six different samples (1, 2, 3, 4, 6 and 14 week-old carcasses) and found that sequences representing the phylum Firmicutes predominated. The diversity of bacterial 16S rRNA gene sequences in the leachate was the highest at 6 weeks, in contrast to those at 2 and 14 weeks. The relative abundance of Firmicutes was reduced, while the proportion of Bacteroidetes and Proteobacteria increased from 3–6 weeks. The representation of phyla was restored... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pig; Decomposition; Leachate; Bacterial community; Pyrosequencing. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000300373 |
| |
|
|
| Band,Guilherme de Oliveira; Guimarães,Simone Eliza Facioni; Lopes,Paulo Sávio; Peixoto,Jane de Oliveira; Faria,Danielle Assis; Pires,Aldrin Vieira; Figueiredo,Frederico de Castro; Nascimento,Carlos Souza do; Gomide,Lúcio Alberto de Miranda. |
| The PSS genotypes of 596 F2 pigs produced by initial mating of Brazilian native boars commercial sows and were characterized by PCR-RFLP and their carcass and performance traits were evaluated. Among the 596 animals analyzed, 493 (82.72%) were characterized as NN and 103 (17.28%) as Nn. With respect to carcass traits, Nn animals presented higher (p < 0.05) right half carcass weight, left half carcass weight, loin depth and loin eye area, and lower shoulder backfat thickness, backfat thickness between last and next to last but one lumbar vertebrae and backfat thickness after last rib at 6.5 cm from the midline compared to NN animals. Nn animals also showed (p < 0.05) higher values for most of the cut yields, indicating higher cutting yields for... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Carcass; Performance; PSS gene; PCR-RFLP; Pig. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100016 |
| |
|
|
| Yonggang,Liu; Sumei,Zhao; Hongbin,Pan; Shizheng,Gao. |
| An investigation of differences in gene expression in the longissimus muscle of Meishan and Large White pigs was undertaken, using the mRNA display technique. A fragment of one differentially expressed gene was isolated and sequenced, whereupon the complete cDNA sequence was then obtained by using the rapid amplification of cDNA ends (RACE). The nucleotide sequence of the gene is not related to any known porcine gene. Sequence analysis revealed that the open reading frame of this gene encodes a protein with 322 amino acids, thus displaying high sequence identity with the PDZ binding kinase (PBK) of eleven other animal species - dog, horse, cattle, human, chimpanzee, crab-eating macaque, rhesus monkey, rat, mouse, gray short-tailed opossum and platypus, so... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pig; PBK; MRNA differential display; RACE. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400017 |
| |
|
|
| Franco,Maurício Machaim; Almeida,Juliana Franco; Souza,Guilherme Rocha Lino de; Antunes,Robson Carlos; Goulart,Luiz Ricardo. |
| After the advent of the genome projects, followed by the discovery of DNA polymorphisms, basic understanding of gene expression is the next focus to explain the association between polymorphisms and the level of gene expression, as well as to demonstrate the interaction among genes. Among the various techniques for the investigation of transcriptional profiling involving patterns of gene expression, quantitative PCR is the simplest analytical laboratory technique. The objective of this work was to analyze two strategies of a competitive PCR technique for the quantification of the pig growth hormone (GH) gene expression. A pair of primers was designed targeting exons 3 and 5, and two competitive PCR strategies were performed, one utilizing a specific... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pig; GH gene; Gene expression; RT-PCR. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100003 |
| |
|
| |
|
|
| Sanchis,E. G; Cristofolini,A. L; Taglialegna,A; Merkis,C. I. |
| The molecules that constitute the extracellular matrix are important in several functions related to tissue support and cell-cell, cell-extracellular matrix interaction. Among the macromolecules that constitute the mentioned matrix we find osteopontin, fibrinogen and collagen. The present study was undertaken to analyze the rol of osteopontin, fibrinogen and collagen in uterine-placental interface during normal porcine gestation. Uterine and placental tissues from crossbred gilts of 30 (n=5), 60 (n=5), 70 (n=5) and 114 (at term, n=5) days of gestation were used. Macroscopic analysis of the embryos/fetuses allowed us to determine their gestational age by means of the crown-rump lenght. Haematoxylin-Eosin and Masson's Trichrome dyes along with light... |
| Tipo: Journal article |
Palavras-chave: Pig; Placenta; Osteopontin; Fibrinogen; Collagen. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000400062 |
| |
|
| |
|
|
| Hou,Qihang; Xia,Mi; Hu,Wiwei; Yu,Qinghua; Zhang,Bingkun. |
| Reducing nitrogen nutrients concentration in dairy food is economic for pig industry. Here, we used finishing pig as model to investigate the effect on colon mucosal barrier and nutrients absorption after reducing crude protein (CP) in dietary from 16 % to 13 %. The results showed that crypt depth, cells, claudin-1 and E-cadherin expression level will not be changed, which implied the integrity of colon mucosal structure. Furthermore, the expressions of ASCT1, EAAT3 and SGLT1 in colon were also maintained at normal levels in 13 % CP dietary. Interestingly, the CAT1 and GLUT2 expression were increased significantly after reducing CP level to 13 %, which might be attributed to the compensatory nutrients absorption. This study implied that 13 % CP was... |
| Tipo: Journal article |
Palavras-chave: Amino acid transporter; Pig; Colon; Goblet cell; Tight junction. |
| Ano: 2017 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000300013 |
| |
|
|
| Monteiro,Adilson; Smith,Ricardo Luiz. |
| The anatomy of mammal's lung air space constitutes the bronchial tree which disposition is associated to air flux dynamics. Casts obtained from human, pig and rat lungs were studied to analyze possible differences of the bronchial tree architecture in mammals with diverse dimensions and posture. Air spaces were filled with polymers through trachea followed by acid corrosion. Tracheal and main bronchial division's diameters were measured to relate with body mass using allometry. The results revealed a dichotomic bronchial branching pattern in the human casts and a monopodial pattern in animals. In allometric relationship trachea was larger in rats, then pigs and lastly in humans, differences were statistically significant, the same occurs in right bronchus,... |
| Tipo: Journal article |
Palavras-chave: Bronchia; Anatomy; Human; Pig; Rat. |
| Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000100050 |
| |
|
|
| Graziotti,Guillermo H; Ríos,Clara M; Rodríguez Menéndez,José M; Salinas,Matías A; Bosco,Alexis; Paltenghi Ceschel,Alejandra; Affricano,Néstor O; Victorica,Carlos L. |
| Neuromuscular compartments are subvolumens supplied by a primary nerve branch with homogeneous fiber characteristics. This muscular organization is important for understanding the muscle function in relation to postural and dynamic implications and also is relevant for appraising meat quality. Current researches associate fiber muscle characteristics with perimortem processes that regulate muscle transformation into meat. It is known that pig muscle has four heavy chain myosin isoforms: I, IIa, IIx and IIb. Castrated male pigs were slaughtered at 100 kg live weight. Semitendinosus muscle samples of each subvolumen determined previously (R1, R2, R3, R4) were frozen in liquid nitrogen and reacted for myofibrillar ATPase and NADH-TR for determining fiber... |
| Tipo: Journal article |
Palavras-chave: Pig; Semitendinosus muscle; Muscle fibers; Meat quality. |
| Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000300048 |
| |
|
|
| Gómez,F. A; Ballesteros,L. E. |
| A few studies conducted on the morphology of coronary arteries in pigs emphasize the description of its course and number of branches. The objective of this study was to determine the anatomic expression of the right coronary artery (RCA) in pigs of commercial stock. One hundred and fifty eight hearts obtained from pigs destined to sacrifice were studied. The RCA were perfused with polyester resin (Palatal 85% and Styrene 15%) and then subjected to KOH infusion for 5 minutes to release the subepicardial fat. Diameters were measured and courses were evaluated. Results: The diameter of the RCA in its proximal portion was 3.85 mm (SD 0.75). The right artery of the cone originated from the RCA in 100 specimens (63.3%) and from the aorta (third coronary) in 8... |
| Tipo: Journal article |
Palavras-chave: Pig; Right coronary artery; Sinoatrial node branch; Right branch of the cone. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000400023 |
| |
| Registros recuperados: 64 | |
|
|
|
