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	Provedor de dados BJM (1) Autor Conceição,Fabrício Rochedo (1) Dellagostin,Odir Antonio (1) Gil-Turnes,Carlos (1) Palavra-chave Escherichia coli K 88ab (1) Fae G (1) Genetic immunization (1) Plasmid production (1) Tipo do documento Info:eu-repo/semantics/article (1) Ano 2001 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última PRODUCTION OF PCB01, A PLASMID FOR DNA IMMUNIZATION AGAINST THE ADHESIN OF ESCHERICHIA COLI K88AB BJM Gil-Turnes,Carlos; Conceição,Fabrício Rochedo; Dellagostin,Odir Antonio. Growth characteristics and plasmid yields of Escherichia coli JM109 transformed with pCB01, a plasmid that encodes genes for the fimbrial adhesin of E. coli K88ab and for ampicillin resistance, grown in two culture media in agitated flasks and in fermentor, are reported. The rate of plasmid loss during growth was estimated by the differential counts in media with and without ampicillin. Plasmid yields of cultures grown in flasks varied from 0.9 to 67 µg/ml of medium, while those grown in fermentor attained 62 µg/ml of medium after 8 hours of culture. Plasmid bearing cells were outgrown by plasmid free cells in proportions varying from 5 to 1.2 non-transformed for each transformed cell during growth. Generation times of total population and plasmid bearing... Tipo: Info:eu-repo/semantics/article Palavras-chave: Plasmid production; Genetic immunization; Fae G; Escherichia coli K 88ab. Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000300012 Registros recuperados: 1 Primeira ... 1 ... Última

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