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Candida albicans morphologies revealed by scanning electron microscopy analysis BJM
Staniszewska,M.; Bondaryk,M.; Swoboda-Kopec,E.; Siennicka,K.; Sygitowicz,G.; Kurzatkowski,W..
Scanning electron microscope (SEM) observations were used to analyze particular morphologies of Candida albicans clinical isolate (strain 82) and mutants defective in hyphae-promoting genes EFG1 (strain HLC52) and/ or CPH1 (strains HLC54 and Can16). Transcription factors Efg1 and Cph1 play role in regulating filamentation and adhesion of C. albicans' morphologies. Comparative analysis of such mutants and clinical isolate showed that Efg1 is required for human serum-induced cell growth and morphological switching. In the study, distinct differences between ultrastructural patterns of clinical strain's and null mutants' morphologies were observed (spherical vs tube-like blastoconidia, or solid and fragile constricted septa vs only the latter observed in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Candida albicans; Pleomorphism; Ultrastructure; Virulence.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300023
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Morphology of endothelial cells from different regions of the equine cornea Ciência Rural
Faganello,Cláudia Skilhan; Silva,Vanessa Ruiz Moura da; Andrade,Maria Cristina Caldart de; Carissimi,André Silva; Pigatto,João Antonio Tadeu.
ABSTRACT: The objective of this study was to evaluate the morphology of different regions of the equine cornea using optical microscopy. Both healthy eyes of eight horses, male or female, of different ages were evaluated. Corneas were stained with alizarin red vital dye and subsequently examined and photographed using optical microscopy. Corneal endothelial morphology of central, superior, inferior, temporal and nasal areas was assessed. One hundred endothelial cells from each corneal area were analyzed. The shape of the corneal endothelial cells of each corneal region was analyzed. Statistical data analysis was conducted using the Student's t test. Values of P<0.01 were considered significant. Regarding morphological analysis, no statistically...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Horses; Corneal endothelium; Pleomorphism; Alizarin red.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001202223
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Morphology of endothelial cells from different regions of the cornea of dogs Ciência Rural
Hünning,Paula Stieven; Andrade,Maria Cristina Caldart de; Carissimi,André; Pigatto,João.
ABSTRACT: The aim of this study was to evaluate the morphology of endothelial cells from different areas of the cornea of dogs. Twenty healthy eyes from 10 dogs, females or males, of different ages were studied. Corneal endothelium morphology of superior, inferior, central, nasal and temporal areas was assessed by 0.2% alizarin red staining using an optic microscope. One hundred endothelial cells from each corneal area were analyzed. In all areas of the cornea studied were found endothelial cells with four sides, five sides, six sides and seven sides. There was no significant difference regarding endothelial cell morphology in all corneal regions evaluated. Thus, the morphology of the central cornea area represents the entire endothelial mosaic and may be...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Canine; Cornea; Endothelium; Alizarin red; Pleomorphism.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001000605
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