|
|
|
| Registros recuperados: 190 | |
|
| |
|
|
| Miranda,Kássia Marques Corrêa; Guimarães,Rejane Araújo; Silva,Marcos José da; Oliveira,Patrícia Rasteiro Ordiale; Ribeiro,Thania Gonçalves; Mendes,Thainara Policarpo; Telles,Mariana Pires de Campos; Soares,Thannya Nascimento. |
| ABSTRACT The genus Manihot Mill. contains about 120 species of which about 104 occur in Brazil. We tested the cross-amplification of ten microsatellite markers developed for Manihot esculenta in 15 species of Manihot endemic to the Brazilian Cerrado. We also evaluated the genetic diversity of Manihot irwinii, M. orbicularis, and M. purpureocostata. Ten pairs of primers were amplified among 14 species of Manihot. The percentage of polymorphic loci per species varied from 70 to 100 %. Nine markers showed amplification and polymorphism when evaluated on polyacrylamide gel. The markers were combined to form three sets for multiplex genotyping for genetic diversity analysis, and showed 51, 75, and 75 alleles in M. irwinii, M. orbicularis, and M.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genetic diversity; Polymorphism; SSR; Transferability wild cassava. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062020000400772 |
| |
|
|
| Pati,Prakash G; Datta,Subhojit; Agbagwa,Ikechukwu O; Singh,Indra Prakash; Soren,Khela Ram; Das,Alok; Choudhary,Arbind Kumar; Chaturvedi,Sushil Kumar. |
| Resistance gene analog (RGA)-anchored amplified fragment length polymorphism (AFLP-RGA) marker system was used in order to evaluate genetic relationships among 22 pigeon pea genotypes with varied responses to Fusarium wilt and sterility mosaic disease. Five AFLP-RGA primer combinations (E-CAG/wlrk-S, M-GTG/wlrk-S, M-GTG/wlrk-AS, E-CAT/S1-INV and E-CAG/wlrk-AS) produced 173 scorable fragments, of which 157 (90.7%) were polymorphic, with an average of 31.4 fragments per primer combination. The polymorphism rates obtained with the five primers were 83.3%, 92.0%, 92.3%, 93.0% and 93.1%, respectively. Mean polymorphic information content (PIC) values ranged from 0.24 (with E-CAT/S1-INV) to 0.30 (with E-CAG/wlrk-AS), whereas resolving power (RP) values varied... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: AFLP-RGA; Cajanus cajan; Genetic diversity; Molecular markers; Polymorphism. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062014000200007 |
| |
|
| |
|
| |
|
|
| Santos, Michele Rocha dos; Universidade Estadual de Maringá; Renesto, Erasmo; Universidade Estadual de Maringá. |
| The genetic variability of Oligosarcus paranensis was estimated from a population collected in São Francisco river, Prudentópolis county in Paraná State (Brazil) using the electrophoresis in starch gel technique. Eleven enzymatic systems were analyzed: Aspartate aminotransaminase (AAT; E. C. 2.6.1), Alcohol dehydrogenase (ADH; E. C. 1.1.1.1), Esterase (EST; E. C. 3.1.1.1), Glucose-6-phosphate isomerase (GPI; E. C. 5.3.1.9), Glycerol-3-Phosphate dehydrogenase (G3PDH; E. C. 1.1.1), Isocitrate dehydrogenase (IDH; E. C. 1.1.1.42), L-lactate dehydrogenase (LDH; E. C. 1.1.1.27), Malate dehydrogenase (MDH; E. C. 1.1.1.37 ), Malate dehydrogenase NADP (ME; E. C. 1.1.1.40), Phosphoglucomutase (PGM; E. C. 5.4.2.2) and Sorbitol dehydrogenase (SORB; E.C. 1.1.1.14).... |
|
Palavras-chave: Allozyme; Genetic diversity; Heterozygosity; Polymorphism; Pisces. |
| Ano: 2013 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/14179 |
| |
|
|
| Tavares,R.A; Nunes,M.D; Almeida,D.B; Silva,J.C; Vaz,B.S; Moreira,C.G.A; Dionello,N.J.L; Piedras,S.R.N; Moreira,H.L.M. |
| Foram identificadas a divergência e a variabilidade genética, por meio do polimorfismo de seis marcadores microssatélites, de duas populações de Odontesthes bonariensis, utilizadas em manejos de cultivo e com potencial para fornecimento de reprodutores para programas de melhoramento genético. Do total de seis loci, cinco demonstraram eficiência para análise genética nas duas populações de O. bonariensis. A diferenciação genética significante nas populações analisadas pode fornecer a base para futuros programas de melhoramentos genéticos, através da combinação de material das populações divergentes para o desenvolvimento de linhagens ou execução de um programa de seleção. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fish; Genetic variability; Polymorphism. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000500034 |
| |
|
|
| Felipe,Aledson V; Silva,Tiago D; Pimenta,Célia A; Kassab,Paulo; Forones,Nora M. |
| BACKGROUND: Studies have demonstrated that some polymorphisms in different interleukin genes may increase the risk of cancer. The aim of this study was to investigate the association between the IL-8 (rs4073) -251A/T gene polymorphism and the risk of gastric cancer (GC). PATIENTS AND METHODS: A case-control study was conducted on patients with noncardia gastric cancer. DNA was extracted from leukocytes and the IL-8 (rs4073) -251A/T polymorphism was analyzed by PCR-RFLP. Infection with Helicobacter pylori was investigated in the serum by ELISA. RESULTS: The sample consisted of 104 patients with GC and 196 controls. Cigarette smoking (P=0.007) and high fat intake (P=0.01) were more frequent in patients with GC. The proportion of patients infected with H.... |
| Tipo: Journal article |
Palavras-chave: IL-8; Polymorphism; Gastric cancer. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000400007 |
| |
|
| |
|
|
| Zheng,Huan; Xu,Huifeng; Cui,Bin; Xie,Nanzi; Wang,Zhi; Luo,Ming. |
| Purpose: The G-protein β3-subunit gene C825T polymorphism (GNB3-C825T) has been reported to be associated with essential hypertension (EH), but results from previous studies are conflicting. The present study aimed at investigating the association between this polymorphism and risk of EH using a meta-analysis on the published studies. Materials and Methods: PubMed, Embase, CBM (China Biological Medicine Database), Wanfang and VIP databases were searched to identify eligible studies published in English and Chinese before March 2013. Data were extracted using standardized methods. The association was assessed by the odds ratio (OR) with 95% confidence intervals (CI). Begg's test was used to measure publication bias. Results: A total of 40... |
| Tipo: Journal article |
Palavras-chave: GTP-Binding Proteins; Polymorphism; Genetic hypertension; Meta-Analysis. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000300007 |
| |
|
| |
|
|
| Valenzuela,Carlos Y. |
| The Neutral Theory of Evolution (NTE) proposes mutation and random genetic drift as the most important evolutionary factors. The most conspicuous feature of evolution is the genomic stability during paleontological eras and lack of variation among taxa; 98% or more of nucleotide sites are monomorphic within a species. NTE explains this homology by random fixation of neutral bases and negative selection (purifying selection) that does not contribute either to evolution or polymorphisms. Purifying selection is insufficient to account for this evolutionary feature and the Nearly-Neutral Theory of Evolution (N-NTE) included negative selection with coefficients as low as mutation rate. These NTE and N-NTE propositions are thermodynamically (tendency to random... |
| Tipo: Journal article |
Palavras-chave: Fixation; Loss; Neutral evolution; Polymorphism; Randomness; Selectiveness. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000200001 |
| |
|
|
| JARA,LILIAN; AMPUERO,SANDRA; SANTIBÁÑEZ,EUDOCIA; SECCIA,LORENA; RODRÍGUEZ,JUAN; BUSTAMANTE,MARIO; LAY-SON,GUILLERMO; OJEDA,JOSÉ MANUEL; REYES,JOSÉ MIGUEL; BLANCO,RAFAEL. |
| BRCA1 gene mutations account for nearly all families with multiple cases of both early onset breast and/or ovarian cancer and about 30% of hereditary breast cancer. Although to date more than 1,237 distinct mutations, polymorphisms, and variants have been described, several mutations have been found to be recurrent in this gene. We have analyzed 63 Chilean breast/ovarian cancer families for eighteen frequent BRCA1 mutations. The analysis of the five exons and two introns in which these mutations are located was made using mismatch PCR assay, ASO hybridization assay, restriction fragment analysis, allele specific PCR assay and direct sequentiation techniques. Two BRCA1 mutations (185delAG and C61G) and one variant of unknown significance (E1250K) were found... |
| Tipo: Journal article |
Palavras-chave: BRCA1 gene; Frequent mutations; Polymorphism; 4185delCAAG; New mutation; Chilean population. |
| Ano: 2004 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000300011 |
| |
|
|
| Alanazi,Mohammad; Pathan,Akbar Ali Khan; Ajaj,Sana Abdulla; Khan,Wajahatullah; Shaik,Jilani P; Al Tassan,Nada; Parine,Narasimha Reddy. |
| DNA repair is one of the central defense mechanisms against mutagenic exposures. Inherited SNPs of DNA repair genes may contribute to variations in DNA repair capacity and susceptibility to cancer. Due to the presence of these variants, inter-individual and ethnic differences in DNA repair capacity have been established in various populations. Saudi Arabia harbors enormous genetic and cultural diversity. In the present study we aimed to determine the genotype and allele frequencies of XRCC1 Arg399Gln (rs25487), XRCC3 Thr241Met (rs861539), XPD Lys751Gln (rs13181), and OGG1 Ser326Cys (rs1052133) gene polymorphisms in 386 healthy individuals residing in the central region of Saudi Arabia and compare them with HapMap and other populations. The genotype and... |
| Tipo: Journal article |
Palavras-chave: DNA Repair genes; Polymorphism; Saudi population; XRCC1; XRCC3; OGG1; XPD. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000200007 |
| |
|
|
| GONZÁLEZ,RODRIGO; TIRADO,ARES; BALANDA,MONSERRAT; ALVO,MIRIAM; BARQUÍN,INÉS; DURRUTY,PILAR; LOBOS,SERGIO; SEELENFREUND,DANIELA. |
| Diabetic nephropathy (DN) is one of the major complications of type 2 diabetes and is associated with coronary disease. Nephrin, a protein mainly expressed in glomeruli, is decreased in DN and other kidney diseases. Since insulin levels are misregulated in type 2 diabetes, a possible connection between DN and its decreased nephrin expression could be the presence of regulatory elements responsive to insulin in the nephrin gene (NPHS1) promoter region. In this work, using bioinformatic tools, we identified a purine-rich GAGA element in the nephrin gene promoter and conducted a genomic study in search of the presence of polymorphisms in this element and its possible association with DN in type 2 diabetic patients. We amplified and sequenced a 514 bp promoter... |
| Tipo: Journal article |
Palavras-chave: GAGA box; Gene promoter; Nephrin; Polymorphism. |
| Ano: 2007 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000400010 |
| |
|
| |
|
| |
|
|
| Souza-Firmino,Tatiani Seni de; Alevi,Kaio Cesar Chaboli; Pereira,Luis Lenin Vicente; Banho,Cecilia Artico; Silva Junior,Fernando Cesar; Souza,Emi Rosane Silistino de; Itoyama,Mary Massumi. |
| In the present paper, 27 chromatic patterns of the specie Pachycoris torridus (Scopoli, 1772) were grouped and three new patterns are described. Because of this high phenotypic polymorphism, P. torridus already been registered eight times as a new specie, highlighting the importance of the application of different tools to assist in taxonomy of this hemipterous of economic importance. |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Insect; Heteroptera; Polymorphism; Agricultural pest. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032016000100406 |
| |
|
| |
| Registros recuperados: 190 | |
|
|
|
