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Genetic diversity of cultivated accessions and wild species of rubber tree using EST‑SSR markers PAB
Perseguini,Juliana Morini Küpper Cardoso; Romão,Lineu Roberto de Castro; Briñez,Boris; Scaloppi Junior,Erivaldo José; Gonçalves,Paulo de Souza; Benchimol,Luciana Lasry.
The objective of this work was to evaluate the efficiency of EST‑SSR markers in the assessment of the genetic diversity of rubber tree genotypes (Hevea brasiliensis) and to verify the transferability of these markers for wild species of Hevea. Forty‑five rubber tree accessions from the Instituto Agronômico (Campinas, SP, Brazil) and six wild species were used. Information provided by modified Roger's genetic distance were used to analyze EST‑SSR data. UPGMA clustering divided the samples into two major groups with high genetic differentiation, while the software Structure distributed the 51 clones into eight groups. A parallel could be established between both clustering analyses. The 30 polymorphic EST‑SSRs showed from two to ten alleles and were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fingerprinting analysis; Genetic structure; Functional molecular markers; Polymorphism information content; Transferability.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2012000800008
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Genetic diversity in soybean germplasm identified by SSR and EST-SSR markers PAB
Mulato,Bruno Mello; Möller,Milene; Zucchi,Maria Imaculada; Quecini,Vera; Pinheiro,José Baldin.
The objectives of this work were to investigate the genetic variation in 79 soybean (Glycine max) accessions from different regions of the world, to cluster the accessions based on their similarity, and to test the correlation between the two types of markers used. Simple sequence repeat markers present in genomic (SSR) and in expressed regions (EST-SSR) were used. Thirty SSR primer-pairs were selected (20 genomic and 10 EST-SSR) based on their distribution on the 20 genetic linkage groups of soybean, on their trinucleotide repetition unit and on their polymorphism information content. All analyzed loci were polymorphic, and 259 alleles were found. The number of alleles per locus varied from 2-21, with an average of 8.63. The accessions exhibit a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycine max; Molecular marker; Plant breeding; Plant germplasm; Polymorphism information content.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2010000300007
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Genetic diversity of Brazilian triticales evaluated with genomic wheat microsatellites PAB
Costa,Cibele Tesser da; Albuquerque,Ana Christina Sagebin; Nascimento Junior,Alfredo do; Marcelino,Francismar Correa; Pereira,Jorge Fernando.
The objective of this work was to determine the genetic variability available for triticale (X Triticosecale Wittmack) crop improvement in Brazil. Forty-two wheat genomic microsatellites were used to estimate the molecular diversity of 54 genotypes, which constitute the base of one of the major triticale breeding programs in the country. Average heterozygosity was 0.06 and average and effective number of alleles per locus were 2.13 and 1.61, respectively, with average allelic frequency of 0.34. The set of genomic wheat microsatellites used clustered the genotypes into seven groups, even when the germplasm was originated primarily from only two triticale breeding programs, a fact reflected on the average polymorphic information content value estimated for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: X Triticosecale; Polymorphism information content; Transferability; Heterozygosity; Number of alleles; Frequency of alleles.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2007001100009
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