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Partial characterization and inactivation of peroxidases and polyphenol-oxidases of umbu-cajá (Spondias spp.) Ciênc. Tecnol. Aliment.
Silva,Cíntia Reis da; Koblitz,Maria Gabriela Bello.
A crude extract of Spondias spp. was evaluated for the influence of pH and temperature on the activity and stability of its peroxidases and polyphenol-oxidases. In order to evaluate the conditions for the inactivation of the enzymes by heat treatment and by addition of a reducing agent, a factorial experimental design (n = 3) was employed using the Statistica (6.0) software package for data analysis. The optimal conditions found for peroxidases were: pH = 5.0 and temperature = 40 ºC, and for polyphenol-oxidases they were pH = 7.0 and temperature = 40 ºC. The peroxidases and polyphenol-oxidases were stable at all pH values tested (3.0 - 10.0) and maintained more than 60% of their activity at temperatures above 30 and 40 ºC, respectively. To achieve the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Peroxidase; Polyphenol-oxidase; Spondias spp.; Inactivation.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612010000300035
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Red clover silage: an alternative for mitigating the impact of nitrogen excretion in ovine production systems R. Bras. Zootec.
Guzatti,Gabriela Cristina; Duchini,Paulo Gonçalves; Kozloski,Gilberto Vilmar; Niderkorn,Vincent; Ribeiro-Filho,Henrique Mendonça Nunes.
ABSTRACT The objective was to quantify the flow of intestinal nutrients and nitrogen excretion and retention in sheep receiving isoproteic diets. Eight Texel x Lacaune wethers (average body weight = 25±2.5 kg) were fitted with duodenal cannula and housed in metabolic cages. Wethers were assigned to the treatments in a crossover design with two periods of 20 days each, and all feces and urine produced by the wethers were collected. The treatments consisted of two isoproteic (160 g kg−1 of crude protein on dry matter basis) diets composed of red clover (RC) or lucerne (LU; Medicago sativa) silages plus corn silage and concentrate feed. The digestible organic matter and metabolizable energy intake did not differ between treatments. The intestinal non-ammonia...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bioactive compounds; Duodenal flow; Polyphenol-oxidase; Quinones; Urinary nitrogen excretion.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982019000100722
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