|
|
|
|
|
| Barbosa,G.H.T.S.; Santana,E.M.; Almeida,A.M.P.; Araujo,A.M.; Fatibello-Filho,O.; Carvalho Jr.,L.B.. |
| F1-antigen purified from Yersinia pestis was covalently linked to 5-mm diameter filter paper discs plasticized with polyvinyl alcohol-glutaraldehyde. These discs were used both for ELISA and dot-ELISA for the detection of anti-F1 IgG in rabbits. The best conditions were achieved using 1.25 µg of F1 antigen/disc, 3% w/v skim milk in PBS as blocking agent, anti-IgG peroxidase conjugate diluted 12,000 times, and serum from rabbits immunized or not against Y. pestis, diluted 6,400 times. The absorbance values obtained from the comparative study between this procedure and conventional ELISA were not significantly different but the low cost of the reagents employed in ELISA using the filter paper discs plasticized with polyvinyl alcohol-glutaraldehyde makes this... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Filter paper; Polyvinyl alcohol; Glutaraldehyde; ELISA; Dot-ELISA; Plague. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000700013 |
| |
|
| |
|
| |
|
|
| Vieira,Rafael Pinto; Fernandes,Alessandra Ribeiro; Kaneko,Telma Mary; Consiglieri,Vladi Olga; Pinto,Claudinéia Aparecida Sales de Oliveira; Pereira,Claudia Silva Cortez; Baby,André Rolim; Velasco,Maria Valéria Robles. |
| Peel off facial masks, based on polyvinyl alcohol (PVA), are formulations that, after application and drying, form an occlusive film over the face. After removing, they provide cleanness, tensor and moisturizing effects, removing dead cells, residues and other materials deposited on the stratum corneous. The soybean extract fermented by Bifidobacterium animalis has sugars, amino acids, peptides, proteins and free isoflavonoids in high concentrations, when compared to the unfermented extract, providing benefits to the cosmetic formulations like anti-aging effect, moisture, tensor action and emollience. The cosmetic bases of peel off facial masks, added with 5.0% w/w of fermented soybean extract, were submitted to Preliminary and Accelerated Stability... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cosmetic formulations/physical and physicochemical stability; Facial mask peel off; Polyvinyl alcohol; Soybean extract; Bifidobacterium animalis/fermentation. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000300018 |
| |
|
|
|
