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| CUNHA,DANIEL A; AMARAL,MARIA EC; CARVALHO,CAROLINA PF; COLLARES-BUZATO,CARLA B; CARNEIRO,EVERARDO M; BOSCHERO,ANTONIO C. |
| During pregnancy and the perinatal period of life, prolactin (PRL) and other lactogenic substances induce adaptation and maturation of the stimulus-secretion coupling system in pancreatic β-cells. Since the SNARE molecules, SNAP-25, syntaxin 1, VAMP-2, and synaptotagmins participate in insulin secretion, we investigated whether the improved secretory response to glucose during these periods involves alteration in the expression of these proteins. mRNA was extracted from neonatal rat islets cultured for 5 days in the presence of PRL and from pregnant rats (17th-18th days of pregnancy) and reverse transcribed. The expression of genes was analyzed by semi-quantitative RT-PCR assay. The expression of proteins was analyzed by Western blotting and... |
| Tipo: Journal article |
Palavras-chave: Insulin; Pancreatic islets; Pregnant rats; Prolactin; SNARE proteins; Synaptotagmin. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000300016 |
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