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	Provedor de dados Scientia Agricola (1) PAB (1) Autor Costa,Alessandra Tenório (1) Freitas,Débora Maria Sansini (1) Müller,Gerd Walter (1) Nunes,William Mário de Carvalho (1) Rezende,Jorge Alberto Marques (1) Zanutto,Carlos Alexandre (1) Mais... Palavra-chave Preimmunization (2) Citrus sinensis (1) Cross protection (1) Cross-protection (1) Cucurbita pepo (1) Potyvirus (1) Single strand conformational polymorphism (1) Stem pitting symptoms (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2010 (1) 2008 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Stability of Citrus tristeza virus protective isolates in field conditions PAB Costa,Alessandra Tenório; Nunes,William Mário de Carvalho; Zanutto,Carlos Alexandre; Müller,Gerd Walter. The objective of this work was to monitor the maintenance of Citrus tristeza virus (CTV) protective isolates stability in selected clones of 'Pêra' sweet orange (Citrus sinensis), preimmunized or naturally infected by the virus, after successive clonal propagations. The work was carried out in field conditions in the north of Paraná State, Brazil. Coat protein gene (CPG) analysis of 33 isolates collected from 16 clones of 'Pêra' sweet orange was performed using single strand conformational polymorphism (SSCP). Initially, the isolates were characterized by symptoms of stem pitting observed in clones. Then viral genome was extracted and used as template for the amplification of CPG by reverse transcription polimerase chain reaction (RTPCR). RTPCR products... Tipo: Info:eu-repo/semantics/article Palavras-chave: Citrus sinensis; Cross-protection; Preimmunization; Single strand conformational polymorphism; Stem pitting symptoms. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2010000700009 Protection between strains of Papaya ringspot virus: type w in zucchini squash involves competition for viral replication sites Scientia Agricola Freitas,Débora Maria Sansini; Rezende,Jorge Alberto Marques. A mild strain of Papaya ringspot virus - type W (PRSV-W), named PRSV-W-1, has proved to be very effective in the control of the disease in zucchini squash plants under greenhouse and field conditions. The aim of this work was to obtain additional information on the protection mechanism between PRSV-W-1 and a severe homologous strain (PRSV-W-C) in Cucurbita pepo L. cv. Caserta. Protective inoculation with the mild strain was made on the cotyledons and the challenge inoculation with the severe strain was applied on the first true expanded leaf, and vice-versa. Plants were challenged at three, six or nine days later, respectively. Plants infected with either the mild or the severe strain alone served as controls. Evaluations were based on the recovery test... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cucurbita pepo; Potyvirus; Cross protection; Preimmunization. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000200012 Registros recuperados: 2 Primeira ... 1 ... Última

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