| Cataractogenesis is a common complication that occurs in diabetes mellitus. Aldose reductase is a lens enzyme probably involved in the development of this eye problem. The purpose of this investigation was to screen plant extracts for aldose reductase inhibitors (ARI) and to investigate their possible influence in diabetic cataractogenesis prevention. 13 plants and 3 natural products were randomly selected for our experiment. The 19 extracts originated from plant material which was extracted with ethanol, water and DCM, and assessed for inhibitors of aldose reductase. This enzyme was isolated from bovine lenses homogenates. The enzyme was incubated in a reaction mixture containing 50 mM Na-phosphate buffer (pH 6.2), NADPH, 400 mM LiSO4 and... |
