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	Provedor de dados Naturalis (1) Scientia Agricola (1) Electron. J. Biotechnol. (1) Genet. Mol. Biol. (1) Autor Alves,Helena Javiel (1) Ceresini,Paulo C. (1) Ciampi,Maisa B. (1) Coutinho,Luiz Lehmann (1) Gale,Liane Rosewich (1) Hoenemann,Claudia (1) Hohe,Annette (1) Hubka, V. (1) Kolarik, M. (1) Ledur,Mônica Corrêa (1) Lemos,Eliana G. de Macedo (1) Ninov,Kerli (1) Nones,Kátia (1) Rosário,Millor Fernandes do (1) Mais... Palavra-chave Primer design (4) Allelic discrimination (1) Biseriate black aspergilli (1) Codon usage (1) Fat deposition (1) Gallus gallus (1) Gene duplication (1) Gene expression analysis (1) In vitro propagation (1) Incongruent trees (1) Multilocus genotyping (1) PCR fingerprinting (1) Paralogous genes (1) Polymorphisms (1) Somatic embryogenesis (1) Uniseriate black aspergilli (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Article / Letter to the editor (1) Journal article (1) Ano 2012 (1) 2011 (1) 2009 (1) 2008 (1) País Brazil (2) Chile (1) Netherlands (1) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 4 Primeira ... 1 ... Última Selection of reference genes for normalization of quantitative real-time PCR in cell cultures of Cyclamen persicum Electron. J. Biotechnol. Hoenemann,Claudia; Hohe,Annette. As a prerequisite for gene expression analyses in cell cultures of the ornamental crop Cyclamen persicum basic parameters for quantitative real-time polymerase chain reaction (qRT-PCR) have been established including the selection of reference genes using the software tools ‘geNorm’ and ‘NormFinder’. Five potential reference genes have been tested (elongation factor tu (Ef-Tu), putative ABC transporter ATPase, putative conserved oligomeric Golgi (COG) complex component, V-ATPase G subunit 1 and Histone H3-K9 methyltransferase 4 (H3-K9-HMTase 4)). ‘NormFinder’ as well as ‘geNorm’ identified Ef-Tu to be the least stable reference gene while the ranking of the most stable genes... Tipo: Journal article Palavras-chave: Gene expression analysis; In vitro propagation; Primer design; Somatic embryogenesis. Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000100012 Distinctively variable sequence-based nuclear DNA markers for multilocus phylogeography of the soybean- and rice-infecting fungal pathogen Rhizoctonia solani AG-1 IA Genet. Mol. Biol. Ciampi,Maisa B.; Gale,Liane Rosewich; Lemos,Eliana G. de Macedo; Ceresini,Paulo C.. A series of multilocus sequence-based nuclear DNA markers was developed to infer the phylogeographical history of the Basidiomycetous fungal pathogen Rhizoctonia solani AG-1 IA infecting rice and soybean worldwide. The strategy was based on sequencing of cloned genomic DNA fragments (previously used as RFLP probes) and subsequent screening of fungal isolates to detect single nucleotide polymorphisms (SNPs). Ten primer pairs were designed based on these sequences, which resulted in PCR amplification of 200-320 bp size products and polymorphic sequences in all markers analyzed. By direct sequencing we identified both homokaryon and heterokaryon (i.e. dikaryon) isolates at each marker. Cloning the PCR products effectively estimated the allelic phase from... Tipo: Info:eu-repo/semantics/article Palavras-chave: Multilocus genotyping; Polymorphisms; Allelic discrimination; Primer design. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400027 β-tubulin paralogue tubC is frequently misidentified as the benA gene in Aspergillus section Nigri taxonomy: primer specificity testing and taxonomic consequences Naturalis Hubka, V.; Kolarik, M.. β-tubulin (benA, tub-2) and calmodulin (caM) are crucial genes in the taxonomy of Aspergillus section Nigri. Widely used β-tubulin primers are not specific for the benA gene for some taxa and preferentially amplify the tubC paralogue. Sequences of the tubC paralogue are widely combined with benA sequences in recent taxonomical works as well as other works, resulting in incongruent trees. In this study we newly provide benA sequences for several ex-type strains, which were characterised using the tubC gene only. We designed a highly specific forward primer to benA designated Ben2f for use in Aspergillus section Nigri, and tested specificity of numerous primer combinations to β-tubulin paralogs. The primer pairs with the highest specificity to the benA gene... Tipo: Article / Letter to the editor Palavras-chave: Biseriate black aspergilli; Codon usage; Gene duplication; Incongruent trees; Paralogous genes; PCR fingerprinting; Primer design; Uniseriate black aspergilli. Ano: 2012 URL: http://www.repository.naturalis.nl/record/531644 Investigation of Leptin gene in broiler and layer chicken lines Scientia Agricola Ninov,Kerli; Ledur,Mônica Corrêa; Alves,Helena Javiel; Rosário,Millor Fernandes do; Nones,Kátia; Coutinho,Luiz Lehmann. Leptin, a polypeptide hormone secreted mainly by adipose tissue, plays an important role in feed intake regulation, energy metabolism and reproduction in several species. Its function has been intensively studied in mammals; however, in birds limited information is available. The cDNA sequence for chicken leptin has been reported, and high hepatic expression levels of leptin were associated with fat deposition in selected bird lines. However, controversies still remain concerning to the chicken leptin gene and several authors failed to amplify this gene from genomic DNA or cDNA. In view of this controversy and the importance of this gene, the present study aimed to investigate the leptin gene in a population of birds developed by Embrapa Swine and Poultry... Tipo: Info:eu-repo/semantics/article Palavras-chave: Gallus gallus; Fat deposition; Primer design. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000200016 Registros recuperados: 4 Primeira ... 1 ... Última

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