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Enhanced production of prodigiosin by Serratia marcescens MO-1 using ram horn peptone BJM
Kurbanoglu,Esabi Basaran; Ozdal,Murat; Ozdal,Ozlem Gur; Algur,Omer Faruk.
This work addresses the production of prodigiosin from ram horn peptone (RHP) using MO-1, a local isolate in submerged culture. First, a novel gram-negative and rod-shaped bacterial strain, MO-1, was isolated from the body of the grasshopper (Poecilemon tauricola Ramme 1951), which was collected from pesticide-contaminated fields. Sequence analysis of 16S rDNA classified the microbe as Serratia marcescens. The substrate utilization potential (BIOLOG) and fatty acid methyl ester profile (FAME) of S. marcescens were also determined. The effect of RHP on the production of prodigiosin by S. marcescens MO-1 was investigated, and the results showed that RHP supplementation promoted the growth of MO-1 and increased the production of prodigiosin. A concentration...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Prodigiosin; Serratia marcescens; Ram horn peptone; Agricultural waste.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200631
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Using prodigiosin against some gram-positive and gram-negative bacteria and Trypanosoma cruzi J. Venom. Anim. Toxins incl. Trop. Dis.
Herráez,Rocío; Mur,Anna; Merlos,Alexandra; Viñas,Miguel; Vinuesa,Teresa.
Abstract Background: This work aimed to explore the action of natural prodigiosin on both bacterial organisms and Trypanosoma cruzi cells. Methods: Natural prodigiosin pigment was extracted and purified from cultures of Serratia marcescens. Two media, peanut broth and peptone glycerol broth, both recommended in the literature for prodigiosin production, were compared. The prodigiosin obtained was employed to explore its antimicrobial properties against both bacteria and Trypanosoma cruzi cells. Results: Peanut broth yielded four times more prodigiosin. The prodigiosin showed remarkable activity (minimal inhibitory concentrations in the range of 2-8 µM for bacteria and half maximal inhibitory concentration of 0.6 µM for Trypanosoma cruzi). In fact, the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Trypanosoma cruzi; Chagas disease; Prodigiosin; Atomic force microscopy.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100314
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