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| Riou, Virginie; Périot, Marine; Biegala, Isabelle. |
| Oligonucleotide probes are increasingly being used to characterize natural microbial assemblages by Tyramide Signal Amplification-Fluorescent in situ Hybridization (TSA-FISH, or CAtalysed Reporter Deposition CARD-FISH). In view of the fast-growing rRNA databases, we re-evaluated the in silico specificity of eleven bacterial and eukaryotic probes and competitor frequently used for the quantification of marine picoplankton. We performed tests on cell cultures to decrease the risk for non-specific hybridization, before they are used on environmental samples. The probes were confronted to recent databases and hybridization conditions were tested against target strains matching perfectly with the probes, and against the closest non-target strains presenting one... |
| Tipo: Text |
Palavras-chave: TSA-FISH; Probes; Specificity; Marine; Prokaryotes; Eukaryotes; Oligonucleotide; CARD-FISH. |
| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00640/75208/75339.pdf |
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| Jebbar, Mohamed; Hickman-lewis, Keyron; Cavalazzi, Barbara; Taubner, Ruth-sophie; Rittmann, Simon K.-m. R.; Antunes, Andre. |
| The icy moons of the outer Solar System harbor potentially habitable environments for life, however, compared to the terrestrial biosphere, these environments are characterized by extremes in temperature, pressure, pH, and other physico-chemical conditions. Therefore, the search for life on these icy worlds is anchored on the study of terrestrial extreme environments (termed “analogue sites”), which harbor microorganisms at the frontiers of polyextremophily. These so-called extremophiles have been found in areas previously considered sterile: hot springs, hydrothermal vents, acidic or alkaline lakes, hypersaline environments, deep sea sediments, glaciers, and arid areas, amongst others. Such model systems and communities in extreme terrestrial environments... |
| Tipo: Text |
Palavras-chave: Extremophiles; Prokaryotes; Metabolism; Diversity; Adaptation; Space explorat. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00606/71771/70275.pdf |
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| Schapira, Mathilde; Mcquaid, Christopher D.; Froneman, Pierre W.. |
| Extensive beds of large subtidal kelps are characteristic of many temperate and subpolar coastlines. They provide habitats for a wide range of other species and are sites of high primary production that generate large quantities of water-borne particles and dissolved organic compounds that support distinctive communities of prokaryotes. We measured prokaryotic metabolism along transects from the shore to the outside of three giant kelp forests (Macrocystis pyrifera) located in the shelf waters of the Prince Edward Islands (Southern Ocean). Abundance, heterotrophic production (PHP), respiration rates (R-ETS) and growth efficiencies (PGE) were investigated within the particle-associated (PA) and the free-living (FL) communities. Temperature, salinity and... |
| Tipo: Text |
Palavras-chave: Prokaryotes; Free-living; Particle-associated; Growth efficiency; Kelp; Sub-Antarctic island. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00088/19939/17760.pdf |
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| Carrier, Vincent; Svenning, Mette M.; Grundger, Friederike; Niemann, Helge; Dessandier, Pierre-antoine; Panieri, Giuliana; Kalenitchenko, Dimitri. |
| Cold seeps are characterized by high biomass, which is supported by the microbial oxidation of the available methane by capable microorganisms. The carbon is subsequently transferred to higher trophic levels. South of Svalbard, five geological mounds shaped by the formation of methane gas hydrates, have been recently located. Methane gas seeping activity has been observed on four of them, and flares were primarily concentrated at their summits. At three of these mounds, and along a distance gradient from their summit to their outskirt, we investigated the eukaryotic and prokaryotic biodiversity linked to 16S and 18S rDNA. Here we show that local methane seepage and other environmental conditions did affect the microbial community structure and composition.... |
| Tipo: Text |
Palavras-chave: Arctic; Methane seeps; Prokaryotes; Methanotrophs; ANME; Sulfate-reducing bacteria; Eukaryotes; Foraminifera. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00667/77893/80077.pdf |
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