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| Yan,Yizhong; Li,Jianjun; Zhang,Yiya; Peng,Xiaozhen; Guo,Tianyao; Wang,Jirong; Hu,Weijun; Duan,Zhigui; Wang,Xianchun. |
| BACKGROUND: Black widow spider (L. tredecimguttatus) has toxic components not only in the venomous glands, but also in other parts of the body and its eggs. It is biologically important to investigate the molecular basis of the egg toxicity. RESULTS: In the present work, an aqueous extract was prepared from the eggs of the spider and characterized using multiple physiological and biochemical strategies. Gel electrophoresis and mass spectrometry demonstrated that the eggs are rich in high-molecular-mass proteins and the peptides below 5 kDa. The lyophilized extract of the eggs had a protein content of 34.22% and was shown to have a strong toxicity towards mammals and insects. When applied at a concentration of 0.25 mg/mL, the extract could completely block... |
| Tipo: Journal article |
Palavras-chave: Black widow spider (L. tredecimguttatus); Protein composition; Egg; Molecular mechanism; Property analysis; Toxicity. |
| Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100017 |
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