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Purification de la chitine par hydrolyse enzymatique à partir de coproduits de crevette Penaeus vannamei. Caractérisations des produits et optimisation du procédé ArchiMer
Le Roux, Karine.
The objective of this study was to optimize the extraction of chitin by acid proteolysis. The novelty of the method is based on the stabilization of pH by the balance between substrate composition and the acid solvent. This principle allows a simultaneous demineralization and deproteinization, the two main reactions associated with the purification of chitin. To evaluate the performance of this purification, the composition of the substrate and products was characterized. Different methods of quantification of chitin and proteins have been compared. As traditional assays were not satisfaying, a direct method of amino acids determination by gaz chromatography was selected to estimate the amount of protein. The estimate of chitin amount was based on indirect...
Tipo: Text Palavras-chave: Coproduits de crustacés; Hydrolyse enzymatique; Protéolyse; Chitine; Extraction; Cinétique; Déminéralisation; Déprotéinisation; Crustacean by-products; Enzymatic hydrolysis; Proteolysis; Chitin; Extraction; Kinetic; Demineralization; Deproteinization.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00087/19828/17470.pdf
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Valorisation de matières premières marines de faible valeur ajoutée : application aux co-produits de thon ArchiMer
Nguyen, Thi-my-huong.
The main objective of this study is the valorisation of by-products coming from processed tuna (Thunnus albacares). Therefore, enzymatic hydrolysis was selected, using an industrial large spectrum protease (Protamex). In a first phase, hydrolyses of these by-products (heads, tails, viscera) were carried out for long periods of time (12h) in order to establish the kinetics of the proteolysis of those different by-products and to obtain the approximate composition of the different resulting fractions and to determine the conditions which would allow the valorisation of these fractions. In a second phase the valorisation in animal nutrition (shrimp culture) and in human nutrition (fish sauce) of the fractions coming from the hydrolysis of the head of tunas...
Tipo: Text Palavras-chave: Feed; Food; Lipids; Peptids; Protamex; Proteolysis; By products; Thuna; Alimentation; Lipides; Peptides; Protamex; Protéolyse; Co produits; Thon.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/these-6918.pdf
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