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| Squaiella-Baptistão,Carla Cristina; Magnoli,Fábio Carlos; Marcelino,José Roberto; Sant’Anna,Osvaldo Augusto; Tambourgi,Denise V.. |
| Abstract Background Among other applications, immunotherapy is used for the post-exposure treatment and/or prophylaxis of important infectious diseases, such as botulism, diphtheria, tetanus and rabies. The effectiveness of serum therapy is widely proven, but improvements on the immunoglobulin purification process and on the quality control are necessary to reduce the amount of protein aggregates. These may trigger adverse reactions in patients by activating the complement system and inducing the generation of anaphylatoxins. Herein, we used immunochemical methods to predict the quality of horse F(ab′)2 anti-botulinum AB, anti-diphtheric, antitetanic and anti-rabies immunoglobulins, in terms of amount of proteins and protein aggregates. Methods Samples... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Heterologous immunoglobulin; F(ab′)2 fragment; Antitoxins; Anti-rabies; Protein profile; Complement system. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100309 |
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| De Vera, M.E; Sato, K; Oyong, G; Claveria, F.G. |
| Fasciola hepatica and Fasciola gigantica habitually co-exist as parasites of cattle (Bos taurus) and water buffalo (Bubalus bubalis), and despite variation in their morphometry, their species status is suspicious. Liver flukes isolated from cattle and water buffalo were initially sorted as F. gigantica or F. hepatica, and crude proteins were extracted and subjected to SDS-PAGE. Cattle had the preponderance of F. gigantica, while F. hepatica was the dominant species in water buffaloes. Co-existing cattle and bubaline fasciolids revealed similar protein profile suggestive of a close genetic relationship. The distinct heavy bands shared by co-existing bubaline F. hepatica and F. gigantica relative to those detected in cattle fasciolids suggests a host... |
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Palavras-chave: Cattle & carabao Fasciola spp; Protein profile; SDS-PAGE; Philippines. |
| Ano: 2009 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2382 |
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| Campos,Célia Maria Teixeira de; Hamad,Antônio José Simões; Amante,Edna Regina; Thapon,Jean-Louis; Nau,Françoise; Guerin-Dubiard,Catherine. |
| This article presents the protein profile in freeze-dried chicken embryo eggs with different development periods (0, 3, 5, 7, 9, 11 days). The protein profile was determined through HPLC-reverse phase, electrophoresis SDS-PAGE and IFE-electrophoresis. Protein profile of these eggs changes according to the period of development and the change is more evident after the 5th day, where there was an increase in ovalbumin, ovotransferrin, apoLDL, apoHDL and lysozyme concentration, and a decrease in ovomucin and ovostatin concentration. The hypothesis that the change in the protein profile is due to protein biosynthesis and disintegration that happens during incubation process is discussed. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Embryo eggs; Protein profile. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962003000700001 |
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| Beltrano,José; Ronco,Marta Guillermina; Arango,María Cecilia. |
| Water deficits cause large yield losses in wheat. Although anthesis is generally considered the most vulnerable period, water deficit during grain filling can also cause yield losses. The objective of this study was to investigate the effect of water stress and rewatering, at three different grain developmental stages, on physiological and grain filling parameters and on yield components. Wheat plants were subjected to water deficit and rewatering at the watery ripe, milk and soft dough stages. In the flag leaf, water stress decreased the relative water content, the chlorophyll and protein content and increased the leakage of solutes, at all three studied grain filling stages. Water stress at the watery ripe and milk stages reduced the final grain dry mass... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Grain filling; Growth rate; Logistic model; Protein profile; Water stress. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000200011 |
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