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	Provedor de dados Anais da ABC (AABC) (1) BABT (1) Autor Basilio,Carmelita A. (1) Carmona,Adriana K. (1) Contiero,Jonas (1) Juliano,Luiz (1) Juliano,Maria Aparecida (1) Monti,Rubens (1) Trevisan,Henrique C. (1) Mais... Palavra-chave Proteolytic enzymes (2) Angiotensin I-converting enzyme (1) Carica papaya (1) Continuous recording assay (1) Cysteine proteinase (1) FRET substrates (1) Fluorescence resonance energy transfer (1) Neprilisin (1) Papain (1) Purification of papain (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2009 (1) 2000 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última The use of Fluorescence Resonance Energy Transfer (FRET) peptidesfor measurement of clinically important proteolytic enzymes Anais da ABC (AABC) Carmona,Adriana K.; Juliano,Maria Aparecida; Juliano,Luiz. Proteolytic enzymes have a fundamental role in many biological processes and are associated with multiple pathological conditions. Therefore, targeting these enzymes may be important for a better understanding of their function and development of therapeutic inhibitors. Fluorescence Resonance Energy Transfer (FRET) peptides are convenient tools for the study of peptidases specificity as they allow monitoring of the reaction on a continuous basis, providing a rapid method for the determination of enzymatic activity. Hydrolysis of a peptide bond between the donor/acceptor pair generates fluorescence that permits the measurement of the activity of nanomolar concentrations of the enzyme. The assays can be performed directly in a cuvette of the fluorimeter or... Tipo: Info:eu-repo/semantics/article Palavras-chave: Continuous recording assay; Fluorescence resonance energy transfer; FRET substrates; Proteolytic enzymes; Angiotensin I-converting enzyme; Neprilisin. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652009000300005 Purification of papain from fresh latex of Carica papaya BABT Monti,Rubens; Basilio,Carmelita A.; Trevisan,Henrique C.; Contiero,Jonas. In the present study we wish to report a method of crystallizing papain from fresh papaya latex which gave higher yields than previously reported. This method does not involve the use of sulphydryl reagents. The papain thus obtained is practically pure and shows a single band when submitted to electrophoresis on polyacrylamide gel, and is identical to the papain obtained by other methods. In routine enzymatic assays, specific activity was measured using Z-gly-pNP and BAEE as substrates. Papain crystallized by this method, without the use of high concentrations of salts or thiol-containing substances such as cysteine and dithiothreitol, is obtained in the form of a complex with natural inhibitors existent in latex which can be removed by dialysis. Tipo: Info:eu-repo/semantics/article Palavras-chave: Papain; Carica papaya; Cysteine proteinase; Proteolytic enzymes; Purification of papain. Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000500009 Registros recuperados: 2 Primeira ... 1 ... Última

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