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	Provedor de dados BJM (2) OAK (1) BABT (1) Scientia Agricola (1) Electron. J. Biotechnol. (1) Autor Amemiya, Yoshimiki (1) Araújo,Elza Fernandes de (1) Campos-Takaki,Galba Maria de (1) Carneiro-da-Cunha,Maria das Graças (1) Christias,Christos (1) Coelho,Irene da Silva (1) Costa,Maurício Dutra (1) Demétrio,Clarice Garcia Borges (1) Hayat,Sikandar (1) Kasuya,Maria Catarina Megumi (1) Koike, Masanori (1) Lima Filho,José Luiz de (1) Mendes,Beatriz Madalena Januzzi (1) Mendes-da-Glória,Fernanda Januzzi (1) Mikami,Koji (1) Mourão Filho,Francisco de Assis Alves (1) Queiroz,Marisa Vieira de (1) Saga,Naotsune (1) Simada, Tohru (1) Takahashi,Megumu (1) Mais... Palavra-chave Protoplast (6) Regeneration (2) Aibo-atrum (1) Alfalfa (1) Allantoin (1) Biotechnology (1) Cell wall components (1) Ceratorhiza (1) Clavulanic acid (1) Cultivar improvement (1) Culture filtrate (1) Epulorhiza (1) Fusion (1) Growth regulator (1) Molecular biology (1) P. yezoensis (1) Pathogenicity and osmotic stabilizer (1) Sclerotium rolfsii (1) Streptomyces clavuligerus (1) Tissue culture (1) Mais... Tipo do documento Info:eu-repo/semantics/article (4) Journal article (1) Ano 2010 (3) 2002 (1) 1999 (1) 1993 (1) País Brazil (4) Chile (1) Japan (1) Idioma Inglês (6)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 6 Primeira ... 1 ... Última Production and regeneration of protoplasts from orchid Mycorrhizal Fungi Epulorhiza repens and Ceratorhiza sp. BABT Coelho,Irene da Silva; Queiroz,Marisa Vieira de; Costa,Maurício Dutra; Kasuya,Maria Catarina Megumi; Araújo,Elza Fernandes de. The aim of this work was to study the standardization of conditions to obtain and regenerate Epulorhiza repens and Ceratorhiza sp. protoplasts. For E. repens, the largest number of protoplasts (8.0 × 10(6) protoplasts/mL) was obtained in 0.6 M KCl, using 15 mg/mL of Lysing Enzymes, and 2-day-old fungal mycelium. When 0.5 M sucrose was used as osmotic stabilizer, the highest frequency of regeneration was achieved (8.5 %); 80.0 % of protoplasts were nucleated, and 20.0 % anucleated. For Ceratorhiza sp., the largest number of protoplasts (4.0 × 10(7) protoplasts/mL) was achieved in 0.6 M NaCl, when 15 mg/mL of Lysing Enzymes and 15mg/mL of Glucanex, with 2-day-old fungal mycelium were used. The highest frequency of regeneration was 6.7 % using 0.5 M sucrose... Tipo: Info:eu-repo/semantics/article Palavras-chave: Ceratorhiza; Epulorhiza; Molecular biology; Protoplast; Regeneration. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100019 Protoplast formation and regeneration from Streptomyces clavuligerus NRRL 3585 and clavulanic acid production BJM Carneiro-da-Cunha,Maria das Graças; Lima Filho,José Luiz de; Campos-Takaki,Galba Maria de. Protoplasts of the wild type Streptomyces clavuligerus NRRL 3585 (ATCC 27064) were formed from spores cultures obtained in the lag, exponential and stationary growth phases by using 0.5% glycine in the culture medium. The protoplasts were obtained by treatment of the cells with lysozyme (EC-3.2.1.17) 40,000 U (1mg/mL), in an osmotic solution for 90 min at 28ºC. The frequency of regenerated protoplasts in the lag phase was 1.7x10³ CFU/mL (28.97%), in the beginning of the exponential phase 0.4x10² CFU/mL (31.67%), in the exponential growth phase 2.5x10³ CFU/mL (46.30%) and 1.0x10(5) CFU/mL in stationary phase (48.45%). Antibiotic production and activity of regenerated protoplasts were observed in all phases, except in the lag phase. The protoplast formation... Tipo: Info:eu-repo/semantics/article Palavras-chave: Streptomyces clavuligerus; Protoplast; Clavulanic acid. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000400014 Isolation and fusion of protoplasts from the phytopathogenic fungus Sclerotium rolfsii (Sacc.) BJM Hayat,Sikandar; Christias,Christos. Sclerotium rolfsii (Sacc.) is a serious plant pathogenic fungus and lacks perfect (basidial) stage in production. Protoplast fusion technology was employed to reconstruct fusants from this fungus. Two strains designated as A and R were used. Maximum protoplast yields of 3.8x10(5) /g mycelia and 2.8x10(5) /g mycelia were formed in strains A and R respectively. Osmotic stabilizer sucrose 1M gave maximum yield. Lysing enzyme at the rate of 15mg/ml was found best for yield. Fusion of protoplasts from strains A and R was carried out in fusion media containing PEG 4000 30% (w/v) with 0.2mM CaCl2. Four fusants F1, F2, F3 and F4 were recovered. Morphological, physiological and pathogenic characters of fusants were compared with parent strains on carrots, beans and... Tipo: Info:eu-repo/semantics/article Palavras-chave: Protoplast; Fusion; Sclerotium rolfsii; Pathogenicity and osmotic stabilizer. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100035 Isolation and regeneration of transiently transformed protoplasts from gametophytic blades of the marine red alga Porphyra yezoensis Electron. J. Biotechnol. Takahashi,Megumu; Uji,Toshiki; Saga,Naotsune; Mikami,Koji. Despite the recent progress of transient gene expression systems in a red alga Porphyra yezoensis by particle bombardment, a stable transformation system has yet to establish in any marine red macrophytes. One of the reasons of the difficulty in genetic transformation in red algae is the lack of systems to select and isolate transformed cells from gametophytic blades. Thus, toward the establishment of the stable transformation system in P. yezoensis, we have developed a procedure by which transiently transformed gametophytic cells were prepared from particle bombarded-gametophytic blade as regeneratable protoplasts. Using mixture of marine bacterial enzymes, yield of protoplasts was high as reported elsewhere; however, these protoplasts did not develop. In... Tipo: Journal article Palavras-chave: Allantoin; Protoplast; P. yezoensis; Regeneration; Transient gene expression. Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000200008 Alfalfa-Verticillium albo-atrum Interactions : IV. Reactions of alfalfa protoplasts to fungal culture filtrates and cell wall components OAK Koike, Masanori; Simada, Tohru; Amemiya, Yoshimiki. 日本草地学会Journal of Japanese Society of Grassland Science 39(1):101-107(1993)より転載 Palavras-chave: Verticillium; Alfalfa; Cell wall components; Protoplast; Aibo-atrum; Culture filtrate. Ano: 1993 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1771 Embryogenic calli induction from nucellar tissue of Citrus cultivars Scientia Agricola Mendes-da-Glória,Fernanda Januzzi; Mourão Filho,Francisco de Assis Alves; Demétrio,Clarice Garcia Borges; Mendes,Beatriz Madalena Januzzi. Nucellar tissues of seven Citrus varieties were introduced onto three growth media to produce embryogenic callus. The media tested were: EME [MT, modified, with the addition of malt extract (500 mg.L-1)]; 1/2-EME [half concentration of MT macronutrients + half concentration of BH3 macronutrients + 500 mg.L-1 malt extract + 1.55 g.L-1 of glutamine]; and EBA [EME + 0.44 muM 6-benzyladenine + 0.04 muM 2,4 D]. Soft friable calli were obtained from 'Cravo' and 'Ponkan' mandarins (Citrus reticulata, Blanco), 'Murcott' tangor (Citrus reticulata Blanco x Citrus sinensis L. Osbeck), 'Serra d'água' and 'Valencia' sweet oranges (Citrus sinensis, L. Osbeck) 120 days after callus induction. 'Natal' and 'Pera' sweet oranges (Citrus sinensis, L. Osbeck) produced hard... Tipo: Info:eu-repo/semantics/article Palavras-chave: Biotechnology; Tissue culture; Cultivar improvement; Growth regulator; Protoplast. Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161999000500012 Registros recuperados: 6 Primeira ... 1 ... Última

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