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| Рябушко, Л. И.; Бесиктепе, С.; Едигер, Д.; Илмаз, Д.; Зенгинер, A.; Рябушко, В. И.; Ли, Р. И.. |
| В Чёрном море в прибрежных водах Крыма впервые обнаружена диатомовая водоросль Pseudo-nitzschia calliantha Lundholm, Moestrup et Hasle, продуцирующая домоевую кислоту. Концентрация токсина в клетках водоросли, культивируемой в питательных средах F/2 и Гольдберга, составляет в среднем соответственно 0.43 и 0.145 пг·кл.-1. Рассмотрены морфология, систематика, экология и токсикологические аспекты вида. |
| Tipo: Journal Contribution |
Palavras-chave: Диатомовая водоросль; Pseudo-nitzschia; Морфология; Токсин; Домоевая кислота; Крым; Чёрное море; Diatom; Morphology; Toxin; Domoic acid; Crimea; Black Sea; Діатомова водорість; Морфологія; Домоєва кислота; Крим; Чорне море. |
| Ano: 2008 |
URL: http://repository.ibss.org.ua/dspace/handle/99011/821 |
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| Lema, Anais Kimberley; Metegnier, Gabriel; Quéré, Julien; Latimier, Marie; Youenou, Agnes; Lambert, Christophe; Fauchot, Juliette; Le Gac, Mickael. |
| Untangling the functional basis of divergence between closely related species is a step towards understanding species dynamics within communities at both the evolutionary and ecological scales. We investigated cellular (i.e: growth, domoic acid production, nutrient consumption) and molecular (transcriptomic analyses) responses to varying nutrient concentrations across several strains belonging to three species of the toxic diatom genus Pseudo-nitzschia. Three main results were obtained. First, strains from the same species displayed similar transcriptomic, but not necessarily cellular, responses to the experimental conditions. It showed the importance of considering intra-specific diversity to investigate functional divergence between species. Second, a... |
| Tipo: Text |
Palavras-chave: Phenotype; Transcriptomics; Pseudo-nitzschia; Divergence; Nutrients. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00482/59392/62157.pdf |
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| Ben Garali, Sondes Melliti; Sahraoui, Ines; Ben Othman, Hiba; Kouki, Abdessalem; De La Iglesia, Pablo; Diogene, Jorge; Lafabrie, Celine; Andree, Karl B.; Fernandez-tejedor, Margarita; Mejri, Kaouther; Meddeb, Marouan; Pringault, Olivier; Hlaili, Asma Sakka. |
| This study investigates the effects of polycyclic aromatic hydrocarbons (PAHs) on two potentially toxic Pseudonitzschia hasleana and P. mannii, isolated from a PAH contaminated marine environment. Both species, maintained in non-axenic cultures, have been exposed during 144 h to increasing concentrations of a 15 PAHs mixture. Analysis of the domoic acid, showed very low concentrations. Dose?response curves for growth and photosynthesis inhibition were determined. Both species have maintained their growth until the end of incubation even at the highest concentration tested (120 ?g l-1), Nevertheless, P mannii showed faster growth and seemed to be more tolerant than P. hasleana. To reduce PAH toxicity, both species have enhanced their biovolume, with a... |
| Tipo: Text |
Palavras-chave: Diatoms; Pseudo-nitzschia; PAHs mixtures; Biodegradation; Eco-toxicology. |
| Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00690/80215/83300.pdf |
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| Martin-jezequel, Veronique; Calu, Guillaume; Candela, Leo; Amzil, Zouher; Jauffrais, Thierry; Sechet, Veronique; Weigel, Pierre. |
| Over the last century, human activities have altered the global nitrogen cycle, and anthropogenic inputs of both inorganic and organic nitrogen species have increased around the world, causing significant changes to the functioning of aquatic ecosystems. The increasing frequency of Pseudo-nitzschia spp. in estuarine and coastal waters reinforces the need to understand better the environmental control of its growth and domoic acid (DA) production. Here, we document Pseudo-nitzschia spp. growth and toxicity on a large set of inorganic and organic nitrogen (nitrate, ammonium, urea, glutamate, glutamine, arginine and taurine). Our study focused on two species isolated from European coastal waters: P. multiseries CCL70 and P. australis PNC1. The nitrogen... |
| Tipo: Text |
Palavras-chave: Pseudo-nitzschia; Toxic diatoms; Nitrogen; Amino acids; Domoic acid. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00308/41912/41188.pdf |
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| Noyer, Charlotte; Abot, Anne; Trouilh, Lidwine; Leberre, Veronique Anton; Dreanno, Catherine. |
| Detection of harmful algal blooms has become a challenging concern because of to the direct impacts on public health and economy. The identification of toxic dinoflagellates and diatoms in monitoring programs requires an extensive taxonomic expertise and is time consuming. Advances in molecular biology have allowed the development of new approaches, more rapid, accurate and cost-effective for detecting these microorganisms. In this context, we developed a new DNA microarray (called, Phytochip) for the simultaneous detection of multiple HAB species with a particular emphasis on Pseudo-nitzschia species. Oligonucleotide probes were designed along the rRNA operon. After DNA extraction, the target rDNA genes were amplified and labeled using an asymmetric PCR;... |
| Tipo: Text |
Palavras-chave: HAB; Pseudo-nitzschia; Microarray; Ribosomal oligonucleotide probes; Environmental monitoring. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00254/36554/42346.pdf |
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| Garvette, Andrea; Nezan, Elisabeth; Badis, Yacine; Bilien, Gwenael; Arce, Paola; Bresnan, Eileen; Gachon, Claire M. M.; Siano, Raffaele. |
| Parasites are key drivers of phytoplankton bloom dynamics and related aquatic ecosystem processes. Yet, the dearth of morphological and molecular information hinders the assessment of their diversity and ecological role. Using single-cell techniques, we characterise morphologically and molecularly, intracellular parasitoids infecting four potentially toxin-producing Pseudo-nitzschia and one Melosira species on the North Atlantic coast. These sequences define two, morphologically indistinguishable clades within the phylum Oomycota, related to the genera of algal parasites Anisolpidium and Olpidiopsis and the diatom parasitoid species Miracula helgolandica. Our morphological data are insufficient to attribute either clade to the still unsequenced genus... |
| Tipo: Text |
Palavras-chave: Pseudo-nitzschia; Diatoms; Marine oomycetes; Plankton parasites; Single-cell analysis; Metabarcoding. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00470/58135/60593.pdf |
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