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| Oh,Chulhong; Nikapitiya,Chamilani; Lee,Youngdeuk; Whang,Ilson; Kang,Do-Hyung; Heo,Soo-Jin; Choi,Young-Ung; Lee,Jehee. |
| An agar-degrading Pseudoalteromonas sp. AG52 bacterial strain was identified from the red seaweed Gelidium amansii collected from Jeju Island, Korea. A β-agarase gene which has 96.8% nucleotide identity to Aeromonas β-agarase was cloned from this strain, and was designated as agaA. The coding region is 870 bp, encoding 290 amino acids and possesses characteristic features of the glycoside hydrolase family (GHF)-16. The predicted molecular mass of the mature protein was 32 kDa. The recombinant β-agarase (rAgaA) was overexpressed in Escherichia coli and purified as a fusion protein. The optimal temperature and pH for activity were 55 ºC and 5.5, respectively. The enzyme had a specific activity of 105.1 and 79.5 unit/mg toward agar and agarose, respectively.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Agar; Aeromonas sp; Β-agarase; Pseudoalteromonas sp; GHF-16; Neoagaro-oligosaccharides. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400006 |
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| Nam,EunSook; Ahn,JongKun. |
| Psychrophilic bacteria, which grow on lactose as a carbon source, were isolated from Antarctic polar sea water. Among the psychrophilic bacteria isolated, strain KNOUC808 was able to grow on lactose at below 5ºC, and showed 0.867 unit of o-nitrophenyl β-D-galactopyranoside(ONPG) hydrolyzing activity at 4ºC. The isolate was gram-negative, rod, aerobic, catalase positive and oxidase positive. Optimum growth was done at 20ºC, pH 6.8-7.2. The composition of major fatty acids in cell of KNOUC801 was C12:0 (5.48%), C12:0 3OH (9.21%), C16:0 (41.83%), C17:0 ω8 (7.24%) and C18:1 ω7 (7.04%). All suthese results together suggest that it is affiliated with Pseudoalteromonas genus. The 16S rDNA sequence corroborate the phenotypic tests and the novel strain was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antarctic marine bacterium; Pseudoalteromonas sp; Cold adapted lactose hydrolyzing enzyme. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300011 |
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