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| Camargo,Carlos Henrique; Bruder-Nascimento,Ariane; Mondelli,Alessandro Lia; Montelli,Augusto Cezar; Sadatsune,Terue. |
| Phenotypic and genotypic SPM and IMP metallo-β-lactamases (MBL) detection and also the determination of minimal inhibitory concentrations (MIC) to imipenem, meropenem and ceftazidime were evaluated in 47 multidrug-resistant Pseudomonas aeruginosa isolates from clinical specimens. Polymerase chain reaction detected 14 positive samples to either blaSPM or blaIMP genes, while the best phenotypic assay (ceftazidime substrate and mercaptopropionic acid inhibitor) detected 13 of these samples. Imipenem, meropenem and ceftazidime MICs were higher for MBL positive compared to MBL negative isolates. We describe here the SPM and IMP MBL findings in clinical specimens of P. aeruginosa from the University Hospital of Botucatu Medical School, São Paulo, Brazil, that... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pseudomonas aeruginosa drug resistance; Bacterial carbapenems metalloproteins poly merase chain reaction. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000500011 |
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| Rodrigues,Ana Claudia Souza; Chang,Marilene Rodrigues; Nóbrega,Gabriela Dorn; Rodrigues,Mayara Souza; Carvalho,Nadia Cristina Pereira; Gomes,Beatriz Garcia; Almeida,Dênio Lopes de; Carvalho,Karyne Rangel; Asensi,Marise Dutra. |
| Infection by Pseudomonas aeruginosa has spread worldwide, with limited options for treatment. The purpose of this study was to investigate metallo-β-lactamase-producing P. aeruginosa strains and compare their genetic profile using samples collected from patients in intensive care units. Forty P. aeruginosa strains were isolated from two public hospitals in Campo Grande, Mato Grosso do Sul State, from January 1st, 2007 to June 31st, 2008. Profiles of antimicrobial susceptibility were determined using the agar diffusion method. Metallo-β-lactamase was investigated using the double-disk diffusion test and PCR. Molecular typing was performed by pulsed-field gel electrophoresis (PFGE). Respiratory and urinary tracts were the most common isolation sites. Of the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pseudomonas aeruginosa drug resistance; Multiple beta-lactamases electrophoresis gel pulsed-field. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000300002 |
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