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The use of rice straw broth as an appropriate medium to isolate purple nonsulfur bacteria from paddy fields Electron. J. Biotechnol.
Nunkaew,Tomorn; Kantachote,Duangporn; Nitoda,Teruhiko; Kanzaki,Hiroshi.
The aims were to explore an appropriate isolating medium for obtaining purple nonsulfur bacteria (PNSB) for use as biofertilizers in saline paddy fields and to obtain pure cultures. We therefore chose a defined isolating medium containing 0.25% NaCl, (Glutamate-Acetate broth, GA) and a rice straw broth to compare them for numbers of PNSB obtained, time to obtain pure cultures, diversity and costs. A total of 30 water and 30 sediment samples were collected from saline paddy fields in southern Thailand and used to isolate PNSB in both the isolating media. Based on 60 samples and a period of 13 days incubation under anaerobic light conditions, a greater number of samples produced PNSB growth in GA broth after only day 3; however, after that the rice straw...
Tipo: Journal article Palavras-chave: Biofertilizer; Isolation; Paddy field; Purple nonsulfur bacteria; Rice straw broth.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000600007
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Isolation of purple nonsulfur bacteria for the removal of heavy metals and sodium from contaminated shrimp ponds Electron. J. Biotechnol.
Panwichian,Saijai; Kantachote,Duangporn; Wittayaweerasak,Banjong; Mallavarapu,Megharaj.
In order to determine whether waters used for the shrimp cultivation contained toxic levels of heavy metals (HMs) and sodium (Na), analysis was carried out on 31 shrimp ponds in areas of southern Thailand. Purple nonsulfur bacteria (PNB) were also isolated from the same ponds to investigate if they could be used for bioremediation of the above contaminants. The highest HMs concentrations of the sediment samples in mg/kg dry weight were found as follows: 0.75 cadmium (Cd), 62.63 lead (Pb), 34.60 copper (Cu) and 58.50 zinc (Zn). However, all sediment samples met Hong Kong standards for dredged sediment. In contrast, contamination of Cu (9-30 µg/L) and Zn (140-530 µg/L) exceeding the standard guidelines for marine aquatic animal set by the Pollution Control...
Tipo: Journal article Palavras-chave: Bioremediation; Heavy metals; Purple nonsulfur bacteria; Salinity; Shrimp farming.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000400003
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