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| Killelea, Tom; Ralec, Celine; Bosse, Audrey; Henneke, Ghislaine. |
| DNA polymerases are versatile tools used in numerous important molecular biological core technologies like the ubiquitous polymerase chain reaction (PCR), cDNA cloning, genome sequencing, and nucleic acid based diagnostics. Taking into account the multiple DNA amplification techniques in use, different DNA polymerases must be optimized for each type of application. One of the current tendencies is to reengineer or to discover new DNA polymerases with increased performance and broadened substrate spectra. At present, there is a great demand for such enzymes in applications, e.g., forensics or paleogenomics. Current major limitations hinge on the inability of conventional PCR enzymes, such as Taq, to amplify degraded or low amounts of template DNA. Besides,... |
| Tipo: Text |
Palavras-chave: DNA polymerase; Archaea; Family D; PCR; Pyrococcus. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00193/30450/28871.pdf |
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| Ralec, Celine. |
| Hyperthermophilic Archaea that thrive under harsh environments (elevated temperature, pH shifts, andionizing radiations) are supposed to be exposed to massive DNA damages. However, the mutations frequencies in hyperthermophilic Archaea are comparable with those of other microorganisms (1) indicating they are equipped with unique and efficient molecular mechanisms to ensure their genome integrity. DNA replication is an essential and conserved process among the three domains of life. DNA polymerases are central enzymes involved in the joining of deoxyribonucleoside 5′-triphosphates (dNTPs) to form the growing DNA chain. In Pyrococcus abyssi (Pab), two familiesDNA polymerases have been described as replicases, one family B (PabPol B) with structural diversity... |
| Tipo: Text |
Palavras-chave: ADN; Archéa; Polymérase; Pyrococcus. |
| Ano: 2013 |
URL: https://archimer.ifremer.fr/doc/00498/60952/64355.pdf |
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