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| Briand, Elisabeth; Salmain, Michèle; Henry, Jean-marie; Perrot, Hubert; Compere, Chantal; Pradier, Claire-marie. |
| Immunosensors, based on the immobilization of a model rabbit antibody on mixed self-assembled monolayers and Protein A as a linking agent on gold transducers, were elaborated and characterized at each step by modulated polarization-infrared spectroscopy (PM-IRRAS) and occasionally by atomic force microscopy (AFM) and quartz crystal microbalance (QCM). By testing two different mixed SAMs comprising 11-mercaptoundecanoic acid (MUA), together with either decanethiol (C9CH3) or mercaptohexanol (C6OH), the role of the chemical composition and structure of the antibody attachment layer upon the sensor performance was demonstrated. (c) 2006 Elsevier B.V. All rights reserved. |
| Tipo: Text |
Palavras-chave: QCM; IRRAS; Thiol attachment; Immunosensor; Mixed SAM' s. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1988.pdf |
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| Briand, Elisabeth; Salmain, M; Compere, Chantal; Pradier, C. |
| Two antibody immobilisation procedures were compared to set up an immunosensor for goat anti-rabbit immunoglobulin (anti-rIgG), i.e. rIgG covalently bound or immobilised via affinity to protein A (PrA). In both cases, the first layer of protein was covalently bound to a mixed self-assembled monolayer (SAM) of mercaptoundecanoic acid (MUA) and mercaptohexanol (C6OH) on a gold surface. The elaboration of the sensitive surfaces, as well as their selectivity and sensitivity were studied step by step by polarization modulation-reflection absorption infra-red spectroscopy (PM-RAIRS) and quartz crystal trticrobalance (QCM) with impedance measurement. QCM measurements showed that the viscoelastic properties of the antibody layer were markedly modified during the... |
| Tipo: Text |
Palavras-chave: RIgG immobilisation; PM RAIRS; QCM; Immunosensors. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3580.pdf |
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| Lazerges M, M; Perrot, H; Zeghib N, N; Antoine, Elisabeth; Compere, Chantal. |
| We report on a probe modification of a quartz crystal microbalance (QCM) DNA-biosensor that permits to reversibly change the DNA sequence detected. A QCM DNA-biosensor was designed by immobilization of a 20-base DNA-disulfide probe on the gold-covered quartz surface of a 27 MHz microbalance (9 MHz, third overtone). After immobilization on the gold covered quartz surface, this probe was modified by hybridization with a 45-base DNA that includes the complementary 20-base sequence, a 5-base spacer and a non-complementary 20-base sequence. The non-complementary sequence constitutes a new probe, different from the DNA-disulfide probe, that permits the detection of a new DNA target. As this 45 bases DNA is changeable by dehybridization, successive different DNA... |
| Tipo: Text |
Palavras-chave: QCM; DNA; Biosensor. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1985.pdf |
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