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| Marques, Thaís Lima; Pinho, Renzo Garcia Von; Pinho, Édila Vilela de Resende Von; Paniago, Bruno da Costa; Freitas, Natália Chagas; Santos, Heloisa Oliveira dos. |
| The objective of this study was to evaluate gene expression related to water deficit tolerance in maize lines. For this, lines previously classified as tolerant (91-T and 32-T) and non-tolerant (24-NT and 57-NT) to water deficit were used. The seeds of the four lines were evaluated for emergence and emergence speed index, and the seedlings were evaluated for root and shoot length under two conditions of water availability (70 and 10% substrate water retention capacity). In transcript analysis, the expression of several genes associated with water deficit tolerance, ZmDBP3, ZmALDH9, ZmAN13, and ZmDREB2A, was evaluated by qRT-PCR for the 91-T and 57-NT lines. It can be concluded that soil water deficiency did not reduce root development. However, the shoot... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Abiotic stress; Zea mays; QRT-PCR.; Water deficit; Genética Vegetal. |
| Ano: 2019 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/43479 |
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| Marques,Thaís Lima; Von Pinho,Renzo Garcia; Von Pinho,Édila Vilela de Resende; Santos,Heloisa Oliveira dos. |
| ABSTRACT Knowing genes related to water deficit is a valuable tool to assist and accelerate the selection process of maize genotypes more adapted to drought conditions. Therefore, this work aimed to evaluate genes expression related to tolerance to water deficit in maize lines, using proteomic and transcriptomic analyses. Two lines previously classified as tolerant (91-T) and non-tolerant (57-NT) to low water availability were used. Dried seeds, ears tips, and seedlings (collected at three, five, and seven days after sowing) of the two maize lines (91-T and 57-NT) were used in proteomic analyses. Seedlings were subject to two contrasting water-availability conditions (70% and 10% of the water retention capacity). The expression of catalase, peroxidase, and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Zea mays; Abiotic stress; Electrophoresis; QRT-PCR.. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542019000100233 |
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