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Biofilm production by clinical isolates of Pseudomonas aeruginosa and structural changes in LasR protein of isolates non biofilm-producing BJID
Lima,Jailton Lobo da Costa; Alves,Lilian Rodrigues; Jacomé,Paula Regina Luna de Araújo; Bezerra Neto,João Pacífico; Maciel,Maria Amélia Vieira; Morais,Marcia Maria Camargo de.
ABSTRACT Introduction: Biofilm production is an important mechanism for the survival of Pseudomonas aeruginosa and its relationship with antimicrobial resistance represents a challenge for patient therapeutics. P. aeruginosa is an opportunistic pathogen frequently associated to nosocomial infections, especially in imunocompromised hosts. Objectives: Analyze the phenotypic biofilm production in P. aeruginosa isolates, describe clonal profiles, and analyze quorum sensing (QS) genes and the occurrence of mutations in the LasR protein of non-biofilm producing isolates. Methods: Isolates were tested for biofilm production by measuring cells adherence to the microtiter plates. Clonal profile analysis was carried out through ERIC-PCR, QS genes were by specific...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas aeruginosa; Biofilm; Multiresistance; Quorum sensing (QS).
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000200129
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